The Effects of Prolactin and Gonadotropin on Luteal Function and Morphology in the Cyclic Golden Hamster
| Autor: | Kazuyoshi Taya, Hisashi Kishi, Gen Watanabe, Motoo Shinoda, Hiroe Kon, Koji Y. Arai |
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| Rok vydání: | 2008 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class media_common.quotation_subject Luteolysis Hamster Apoptosis Estrous Cycle DNA Fragmentation Luteal phase Biology Chorionic Gonadotropin Corpus Luteum Cricetinae Internal medicine medicine Animals Horses Equine chorionic gonadotropin Ovulation Progesterone reproductive and urinary physiology Hypophysectomy media_common Estrous cycle Mesocricetus urogenital system Organ Size Prolactin medicine.anatomical_structure Endocrinology Female Animal Science and Zoology Gonadotropin Corpus luteum hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Reproduction and Development. 54:418-423 |
| ISSN: | 1348-4400 0916-8818 |
| Popis: | The present study was conducted to evaluate the endocrinological effects of the pituitary on luteal maintenance and regression in the cyclic golden hamster (Mesocritus auratus). After hypophysectomy (Hypox) at 0900 h on day 1 of the estrous cycle (the day of ovulation), the animals received injection of prolactin (PRL) or PRL plus equine chorionic gonadotropin (eCG). They were decapitated at 1500 h on day 3 of the cycle, and trunk blood was collected for measurement of progesterone (P4). Corpora lutea (CLs) were dissected from one ovary for DNA ladder detection by electrophoresis, determination of DNA fragmentation ratio by fluorometric measurement method and measurement of P4. The other ovary was used for histological observation. After the Hypox, the daily injection of 1 mg ovine PRL restrained the DNA fragmentation ratio and number of apoptotic cell in the CLs. The PRL treatment maintained the luteal morphology and increased the luteal P4 concentration, but not in the plasma P4 concentration. In addition to PRL, injection of 2 IU eCG after the Hypox also restrained the DNA fragmentation ratio and number of apoptotic cells in the CLs to the level of a pregnant animal. The PRL plus eCG treatment maintained the luteal morphology in the same manner as the PRL only treatment and increased not only the luteal but also the plasma P4 concentration. These results suggest that PRL restrains luteal apoptosis and maintains luteal morphology and that the combination of PRL and eCG restrains not only structural but also functional luteal regression in the cyclic hamster. |
| Databáze: | OpenAIRE |
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