Enzyme feeding strategies for better fed-batch enzymatic hydrolysis of empty fruit bunch
| Autor: | Ronny Purwadi, Hauna Fathmadinda Hosen, Ardiyan Harimawan, Yohanes Eko Chandra Sugiharto, Andry, Rina Mariyana, Made Tri Ari Penia Kresnowati, Hana Nur Fitriana |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Environmental Engineering 020209 energy Bioengineering 02 engineering and technology Arecaceae Lignin 01 natural sciences law.invention chemistry.chemical_compound Hydrolysis Cellulase Magazine Pulmonary surfactant Polysaccharides law 010608 biotechnology Enzymatic hydrolysis 0202 electrical engineering electronic engineering information engineering Food science Cellulose Waste Management and Disposal chemistry.chemical_classification Renewable Energy Sustainability and the Environment Substrate (chemistry) General Medicine Steam Enzyme chemistry Chemical engineering Batch Cell Culture Techniques Fruit Current technology |
| Zdroj: | Bioresource Technology. 207:175-179 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2016.01.113 |
| Popis: | Lignin inhibitory becomes a major obstacle for enzymatic hydrolysis of empty fruit bunch conducted in high solid loading. Since current technology required high enzyme loading, surfactant application could not effectively used since it is only efficient in low enzyme loading. In addition, it will increase final operation cost. Hence, another method namely "proportional enzyme feeding" was investigated in this paper. In this method, enzyme was added to reactor proportionally to substrate addition, different from conventional method ("whole enzyme feeding") where whole enzyme was added prior to hydrolysis process started. Proportional enzyme feeding could increase enzymatic digestibility and glucose concentration up to 26% and 12% respectively, compared to whole enzyme feeding for hydrolysis duration more than 40h. If enzymatic hydrolysis was run less than 40h (25% solid loading), whole enzyme feeding is preferable. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect