Atrial natriuretic peptide stimulates Na+ -dependent Ca2+ efflux from freshly isolated adult rat cardiomyocytes
| Autor: | Kazuo Minakuchi, Tetsuya Kitagawa, Koichiro Tsuchiya, Itsuo Katoh, Hitoshi Houchi, Masanori Yoshizumi, Kazuya Horike, Toshiaki Tamaki |
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| Rok vydání: | 1997 |
| Předmět: |
Natriuretic peptide receptor
Male medicine.medical_specialty medicine.drug_class Biophysics Guanosine Cardiomyocyte Biochemistry Rats Sprague-Dawley chemistry.chemical_compound Atrial natriuretic peptide Structural Biology Internal medicine Genetics Extracellular medicine Natriuretic peptide Animals Receptor Molecular Biology Cells Cultured Ion Transport Chemistry Myocardium Sodium Na+/Ca2+ exchange Ca2+ efflux Cell Biology Guanosine 3′ 5′-cyclic monophosphate Brain natriuretic peptide Rats Endocrinology cardiovascular system Calcium Efflux Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists Intracellular |
| Zdroj: | FEBS Letters. 419:255-258 |
| ISSN: | 0014-5793 |
| Popis: | In the present study, we examined the effect of atrial natriuretic peptide (ANP) on Ca2+ efflux from freshly isolated adult rat cardiomyocytes. Rat ANP(1–28) stimulated the efflux of 45Ca2+ from the cells in a concentration-dependent manner (10−8 M to 10−6 M). The 45Ca2+ efflux was not affected by removal of extracellular Ca2+, but was dependent on the presence of extracellular Na+. In addition, rat ANP(1–28) caused 22Na+ influx into the cells. The 45Ca2+ efflux was also stimulated by C-type natriuretic peptide-22 (CNP-22), but not by rat brain natriuretic peptide-45 (BNP-45). It was also observed that both rat ANP(1–28) and CNP-22 stimulated guanosine 3′,5′-cyclic monophosphate production within the cells. These results indicate that ANP stimulates Na+-dependent 45Ca2+ efflux from freshly isolated adult rat cardiomyocytes, probably through Na+/Ca2+ exchange, and that the stimulatory effect of ANP on Ca2+ efflux may be mediated via the natriuretic peptide receptor which has been shown to couple to guanylate cyclase. Since it is reported that Na+/Ca2+ exchange is important in calcium homeostasis within cells, ANP may play a role in the extrusion of intracellular Ca2+ from isolated adult rat cardiomyocytes. |
| Databáze: | OpenAIRE |
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