A robust sensor platform for label-free detection of anti-Salmonella antibodies using undiluted animal sera
| Autor: | Günther Proll, Günter Gauglitz, Melanie Ewald, Alexander Le Blanc |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Reproducibility
Salmonella Chromatography biology Chemistry Diagnostic marker Biosensing Techniques medicine.disease_cause Antibodies Bacterial Biochemistry Analytical Chemistry Reflectometric interference spectroscopy Surface preparation biology.protein medicine Animals Antibody Biosensor Label free |
| Zdroj: | Analytical and Bioanalytical Chemistry. 405:6461-6469 |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | We present a portable and easy-to-use biosensor platform, allowing for label-free detection of diagnostic markers in undiluted animal serum. Exemplarily, this is shown for the detection of anti-Salmonella antibodies. 1-lambda-Reflectometry was used as detection method, making the new biosensor platform portable, cheap, and robust. As recognition elements, lipopolysaccharides (LPSs) from Salmonella typhimurium bacteria were immobilized as sensitive layer on the transducer to carry out serological tests via a direct assay format. For this purpose, a new surface preparation protocol has been worked out allowing for immobilization of the LPS via hydrophobic interactions. It has been shown that results obtained by 1-lambda-Reflectometry are equivalent to those obtained by the non-portable Reflectometric Interference Spectroscopy setup. The new sensor platform was calibrated in both matrices, buffer and undiluted serum. Good sensitivity, selectivity and intra chip reproducibility have been observed. Furthermore, inter chip reproducibility was examined and recovery rates were found to be between 99 and 117 % in undiluted serum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|