Antivenom Neutralization of Coagulopathic Snake Venom Toxins Assessed by Bioactivity Profiling Using Nanofractionation Analytics

Autor: Chunfang Xie, Ben Bruyneel, Jeroen Kool, Freek J. Vonk, Julien Slagboom, Laura-Oana Albulescu, Govert W. Somsen, Nicholas R. Casewell, Kristina B.M. Still
Přispěvatelé: AIMMS, BioAnalytical Chemistry, Chemistry and Pharmaceutical Sciences
Rok vydání: 2019
Předmět:
Proteomics
Health
Toxicology and Mutagenesis
Antivenom
lcsh:Medicine
Snake Bites
Venom
Pharmacology
Toxicology
snakebite
Neutralization
Mass Spectrometry
Viperidae
Medicine
Bothrops
Snakebite
anticoagulation
nanofractionation
0303 health sciences
Echis ocellatus
biology
Calloselasma rhodostoma
qv_4
Antivenins
030302 biochemistry & molecular biology
3. Good health
Snake venom
wd_410
Viper Venoms
qv_38
Nanofractionation
complex mixtures
Article
03 medical and health sciences
Anticoagulation
SDG 3 - Good Health and Well-being
Animals
Russell's Viper
coagulation
Blood Coagulation
030304 developmental biology
Coagulation
antivenom
Deinagkistrodon acutus
business.industry
lcsh:R
qv_195
biology.organism_classification
High-Throughput Screening Assays
Phospholipases A2
Echis carinatus
Metalloproteases
qy_410
Serine Proteases
business
Peptides
Chromatography
Liquid
Zdroj: Toxins
Xie, C, Slagboom, J, Albulescu, L O, Bruyneel, B, Still, K B M, Vonk, F J, Somsen, G W, Casewell, N R & Kool, J 2020, ' Antivenom neutralization of coagulopathic snake venom toxins assessed by bioactivity profiling using nanofractionation analytics ', Toxins, vol. 12, no. 1, 53, pp. 1-16 . https://doi.org/10.3390/toxins12010053
Volume 12
Issue 1
Toxins, 12(1):53, 1-16. Multidisciplinary Digital Publishing Institute (MDPI)
Toxins, Vol 12, Iss 1, p 53 (2020)
ISSN: 2072-6651
DOI: 10.3390/toxins12010053
Popis: Venomous snakebite is one of the world&rsquo
s most lethal neglected tropical diseases. Animal-derived antivenoms are the only standardized specific therapies currently available for treating snakebite envenoming, but due to venom variation, often this treatment is not effective in counteracting all clinical symptoms caused by the multitude of injected toxins. In this study, the coagulopathic toxicities of venoms from the medically relevant snake species Bothrops asper, Calloselasma rhodostoma, Deinagkistrodon acutus, Daboia russelii, Echis carinatus and Echis ocellatus were assessed. The venoms were separated by liquid chromatography (LC) followed by nanofractionation and parallel mass spectrometry (MS). A recently developed high-throughput coagulation assay was employed to assess both the pro- and anticoagulant activity of separated venom toxins. The neutralization capacity of antivenoms on separated venom components was assessed and the coagulopathic venom peptides and enzymes that were either neutralized or remained active in the presence of antivenom were identified by correlating bioassay results with the MS data and with off-line generated proteomics data. The results showed that most snake venoms analyzed contained both procoagulants and anticoagulants. Most anticoagulants were identified as phospholipases A2s (PLA2s) and most procoagulants correlated with snake venom metalloproteinases (SVMPs) and serine proteases (SVSPs). This information can be used to better understand antivenom neutralization and can aid in the development of next-generation antivenom treatments.
Databáze: OpenAIRE
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