Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity
| Autor: | Pierre-Régis Burgel, Isabelle Sermet-Gaudelus, Louise Jeammet, Gérard Lambeau, Lhousseine Touqui, Dominique Leduc, Michel Chignard, Laurent Guillemot, Clémence Martin, Philippe Morand, Dorota Sands, Yongzheng Wu, Erwan Pernet |
|---|---|
| Přispěvatelé: | Défense innée et inflammation, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cellule Pasteur UPMC, Institut Pasteur [Paris] (IP)-Sorbonne Université (SU), Université Paris Descartes - Paris 5 (UPD5), Service de pneumologie [CHU Cochin], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Service de Pneumologie et d'Allergologie Pédiatriques, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Régulation du transport anionique transépithélial : de la recherche fondamentale aux pathologies épithéliales (UMR_S 806), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), National Research Institute of Mother and Child [Warsaw, Poland], Service de Bactériologie [CHU Cochin, AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], This study was supported by France ‘Vaincre la Mucoviscidose’ and ‘DIM Malinf-Region Ile de France’ foundation., We thank Dr R. Ramphal (University of Florida, Gainesville, FL, USA), Dr T. Msadek (Institut Pasteur, Paris), Dr E. Pearlman (Case Western Reserve University, Cleveland, OH, USA), Dr J.C. Olson (West Virginia University, Morgantown, WV, USA), Dr P. Peris (University of València, Spain), Dr M. Gelb (University of Washington, Seattle, WA, USA) for providing PA and SA strains as well as substrate for sPLA2 activity assay and RO032007A compound, Dr C. Schmitt (Hôpital Trousseau, Paris) for providing expectorations of children patients, V. Balloy and M. Nahori (Institut Pasteur, Paris) for technical assistance with intratracheal and intravenous instillation and C. Payre for helpful advices in sPLA2 analyses., Wu, Yongzheng, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Sorbonne Université (SU), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5) |
| Rok vydání: | 2014 |
| Předmět: |
Cystic Fibrosis
MESH: ADP Ribose Transferases General Physics and Astronomy MESH: Staphylococcus aureus / physiology medicine.disease_cause [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity Cystic fibrosis Mice [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: Child MESH: Respiratory Mucosa MESH: Animals Child ADP Ribose Transferases MESH: Sputum / enzymology 0303 health sciences Multidisciplinary MESH: Bronchi respiratory system 3. Good health MESH: Young Adult Staphylococcus aureus Child Preschool Pseudomonas aeruginosa Disease Progression [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: Disease Progression Adult Host immunity Adolescent Bacterial Toxins Guinea Pigs Bronchi Respiratory Mucosa MESH: Epithelial Cells / enzymology Biology Group II Phospholipases A2 General Biochemistry Genetics and Molecular Biology MESH: Guinea Pigs Microbiology Young Adult 03 medical and health sciences MESH: Pseudomonas aeruginosa / physiology medicine Animals Humans MESH: Mice [SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity 030304 developmental biology MESH: Adolescent Respiratory tract diseases MESH: Humans MESH: Group II Phospholipases A2 / metabolism 030306 microbiology MESH: Child Preschool Sputum Epithelial Cells MESH: Adult General Chemistry medicine.disease [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology respiratory tract diseases MESH: Bacterial Toxins Immunology MESH: Cystic Fibrosis / enzymology Bacterial infection [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Cystic Fibrosis / microbiology |
| Zdroj: | Nature Communications Nature Communications, 2014, 5 (1), pp.5105. ⟨10.1038/ncomms6105⟩ Nature Communications, Nature Publishing Group, 2014, 5 (1), pp.5105. ⟨10.1038/ncomms6105⟩ |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms6105 |
| Popis: | International audience; Young cystic fibrosis (CF) patients' airways are mainly colonized by Staphylococcus aureus, while Pseudomonas aeruginosa predominates in adults. However, the mechanisms behind this infection switch are unclear. Here, we show that levels of type-IIA-secreted phospholipase A2 (sPLA2-IIA, a host enzyme with bactericidal activity) increase in expectorations of CF patients in an age-dependent manner. These levels are sufficient to kill S. aureus, with marginal effects on P. aeruginosa strains. P. aeruginosa laboratory strains and isolates from CF patients induce sPLA2-IIA expression in bronchial epithelial cells from CF patients (these cells are a major source of the enzyme). In an animal model of lung infection, P. aeruginosa induces sPLA2-IIA production that favours S. aureus killing. We suggest that sPLA2-IIA induction by P. aeruginosa contributes to S. aureus eradication in CF airways. Our results indicate that a bacterium can eradicate another bacterium by manipulating the host immunity. |
| Databáze: | OpenAIRE |
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