Longitudinal monitoring reveals dynamic changes in circulating tumor cells (CTCs) and CTC-associated miRNAs in response to chemotherapy in metastatic colorectal cancer patients
| Autor: | Karen Tan, Michael Vito Martin Blanco, Sai Mun Leong, Zizheng Kee, Patrick Vincent Caramat, Thomas I-Peng Soh, Wei Peng Yong, Wai Kit Cheong, James Teo, Evelyn Siew-Chuan Koay, Angela Pang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Oncology Cancer Research medicine.medical_specialty Response to therapy Colorectal cancer medicine.medical_treatment Cell Count Pilot Projects 03 medical and health sciences 0302 clinical medicine Circulating tumor cell Carcinoembryonic antigen Internal medicine Antineoplastic Combined Chemotherapy Protocols microRNA Humans Medicine In patient Longitudinal Studies Prospective Studies Neoplasm Metastasis neoplasms Aged Chemotherapy biology business.industry Disease progression Middle Aged Neoplastic Cells Circulating medicine.disease digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs Treatment Outcome 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Female Colorectal Neoplasms business |
| Zdroj: | Cancer Letters. 423:1-8 |
| ISSN: | 0304-3835 |
| Popis: | We evaluated the changes in CTC count and CTC-associated miRNAs during the course of chemotherapy in patients with metastatic colorectal cancer. Blood samples were collected from 9 metastatic colorectal cancer patients prior to chemotherapy and at every other chemotherapy session during the course of treatment. CTCs were isolated and enumerated using a size-exclusion method (CellSievo, Singapore). CTC-associated miRNAs were isolated using a paper-based, partitioning method, and analyzed using reverse transcription quantitative real-time PCR (MiRXES, Singapore). CTC count trends generally correlated with disease progression defined by radiological measurements and trends in carcinoembryonic antigen (CEA) levels; hence CTC counts may be useful in cases where CEA is not elevated. CTC-associated miRNAs identified were miR-15b, miR-16, miR-19a, miR-21, miR-25, miR-30d, miR-126, miR-185, miR-221, miR-222, and miR-324–5p. The expression of CTC-associated miRNAs did not appear to correlate with CTC count and exhibited inter-individual heterogeneity. This pilot study suggests that analysis of CTC changes during the course of treatment may be useful in monitoring response to therapy in metastatic colorectal cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect