Mitochondrial DNA and temperature tolerance in lager yeasts
| Autor: | Ryan V. Moriarty, Justin C. Fay, Xueying C. Li, David Peris, EmilyClare P. Baker, Chris Todd Hittinger |
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| Přispěvatelé: | European Commission, National Institutes of Health (US), National Institute of Food and Agriculture (US), The Pew Charitable Trusts |
| Rok vydání: | 2019 |
| Předmět: |
Thermotolerance
Mitochondrial DNA Saccharomyces cerevisiae Biology DNA Mitochondrial Saccharomyces 03 medical and health sciences Genetics Research Articles 030304 developmental biology Hybrid 0303 health sciences Multidisciplinary Whole Genome Sequencing Strain (chemistry) Chimera 030306 microbiology Saccharomyces eubayanus technology industry and agriculture Beer SciAdv r-articles food and beverages biology.organism_classification Saccharomyces pastorianus Yeast Cold Temperature Genetic Loci Fermentation Genome Mitochondrial Hybridization Genetic Adaptation Research Article |
| Zdroj: | Science Advances Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.aav1869 |
| Popis: | A growing body of research suggests that the mitochondrial genome (mtDNA) is important for temperature adaptation. In the yeast genus Saccharomyces, species have diverged in temperature tolerance, driving their use in high- or low-temperature fermentations. Here, we experimentally test the role of mtDNA in temperature tolerance in synthetic and industrial hybrids (Saccharomyces cerevisiae × Saccharomyces eubayanus or Saccharomyces pastorianus), which cold-brew lager beer. We find that the relative temperature tolerances of hybrids correspond to the parent donating mtDNA, allowing us to modulate lager strain temperature preferences. The strong influence of mitotype on the temperature tolerance of otherwise identical hybrid strains provides support for the mitochondrial climactic adaptation hypothesis in yeasts and demonstrates how mitotype has influenced the world’s most commonly fermented beverage. This work was supported by the USDA National Institute of Food and Agriculture (Hatch project no. 1003258), the NSF (grant no. DEB-1253634), and in part by the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science; nos. DE-SC0018409 and DE-FC02-07ER64494). E.P.B. was supported by a Louis and Elsa Thomsen Wisconsin Distinguished Graduate Fellowship. C.T.H. is a Pew Scholar in the Biomedical Sciences and a Vilas Faculty Early Career Investigator, supported by the Pew Charitable Trusts and the Vilas Trust Estate. D.P. is a Marie Sklodowska-Curie fellow of the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 747775). J.C.F. was supported by the NIH (no. GM080669) |
| Databáze: | OpenAIRE |
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