Interaction of Peptide Transporter 1 With d-Glucose and l-Glutamic Acid; Possible Involvement of Taste Receptors
| Autor: | Takuo Ogihara, Kaori Morimoto, Taichi Ohmachi, Kiko Ichiba, Yoko Idota, Masahiko Kanagawa, Hiroki Kamioka, Takumi Tomono, Yasuko Hatano, Kentaro Yano, Hiroshi Arakawa |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Nifedipine Sensory Receptor Cells Cmax Glutamic Acid Pharmaceutical Science Peptide Transporter 1 Intestinal absorption 03 medical and health sciences chemistry.chemical_compound D-Glucose Oral administration Internal medicine medicine Animals Mannitol Channel blocker Rats Wistar Cephalexin Membranes Symporters biology Chemistry digestive oral and skin physiology Peptide transporter 1 Glutamic acid Calcium Channel Blockers Taste Buds Rats Protein Transport Glucose Jejunum 030104 developmental biology Endocrinology Intestinal Absorption biology.protein medicine.drug |
| Zdroj: | Journal of Pharmaceutical Sciences. 105:339-342 |
| ISSN: | 0022-3549 |
| DOI: | 10.1016/j.xphs.2015.11.024 |
| Popis: | We investigated the influence of sweet and umami (savory) tastants on the intestinal absorption of cephalexin (CEX), a substrate of peptide transporter 1 (PEPT1, SLC15A1) in rats. After oral administration of glucose or mannitol to rats, CEX was administered together with a second dose of glucose or mannitol. Western blot analysis indicated that expression of PEPT1 in rat jejunum membrane was decreased by glucose, compared to mannitol. Furthermore, the maximum plasma concentration (Cmax) of orally administered CEX was reduced by glucose compared to mannitol. The effect of glucose was diminished by nifedipine, a L-type Ca(2+) channel blocker. We also found that Cmax of orally administered CEX was reduced by treatment with L-glutamic acid, compared to D-glutamic acid. Thus, excessive intake of glucose and L-glutamic acid may impair oral absorption of PEPT1 substrates. |
| Databáze: | OpenAIRE |
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