Substrates of the chloroplast small heat shock proteins 22E/F point to thermolability as a regulative switch for heat acclimation in Chlamydomonas reinhardtii
| Autor: | Frederik Sommer, Timo Mühlhaus, Miriam Schulz-Raffelt, Michael Schroda, Felix Willmund, Juliane Schurig, Sylvia Thoms, Mark Rütgers, Ligia Segatto Muranaka |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Molecular chaperones Chloroplasts Hot Temperature Acclimatization Chlamydomonas reinhardtii Plant Science Biology Protein–protein interactions Genes Plant 01 natural sciences Chloroplast Antibodies Article Substrate Specificity HSPA4 03 medical and health sciences Heat acclimation Heat shock protein Genetics Amino Acid Sequence Phylogeny Mass spectrometry Chlamydomonas Reproducibility of Results General Medicine biology.organism_classification Hsp70 Heat-Shock Proteins Small Molecular Weight 030104 developmental biology Chloroplast DNA Biochemistry Biophysics CLPB Protein homeostasis Agronomy and Crop Science Heat-Shock Response 010606 plant biology & botany |
| Zdroj: | Plant Molecular Biology |
| ISSN: | 1573-5028 |
| Popis: | Key message We have identified 39 proteins that interact directly or indirectly with high confidence with chloroplast HSP22E/F under heat stress thus revealing chloroplast processes affected by heat. Abstract Under conditions promoting protein unfolding, small heat shock proteins (sHsps) prevent the irreversible aggregation of unfolding proteins by integrating into forming aggregates. Aggregates containing sHsps facilitate the access of Hsp70 and ClpB/Hsp104 chaperones, which in ATP-dependent reactions disentangle individual proteins from the aggregates and assist in their refolding to the native state. Chlamydomonas reinhardtii encodes eight different sHsps (HSP22A to H). The goal of this work was to identify chloroplast-targeted sHsps in Chlamydomonas and to obtain a comprehensive list of the substrates with which they interact during heat stress in order to understand which chloroplast processes are disturbed under heat stress. We show that HSP22E and HSP22F are major chloroplast-targeted sHsps that have emerged from a recent gene duplication event resulting from the ongoing diversification of sHsps in the Volvocales. HSP22E/F strongly accumulate during heat stress and form high molecular mass complexes. Using differential immunoprecipitation, mass spectrometry and a stringent filtering algorithm we identified 39 proteins that with high-confidence interact directly or indirectly with HSP22E/F under heat stress. We propose that the apparent thermolability of several of these proteins might be a desired trait as part of a mechanism enabling Chlamydomonas chloroplasts to rapidly react to thermal stress. Electronic supplementary material The online version of this article (doi:10.1007/s11103-017-0672-y) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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