An update on the advancing high-throughput screening techniques for patch clamp-based ion channel screens: implications for drug discovery
| Autor: | Nina Brinkwirth, Andrea Brüggemann, Michael George, Nadine Becker, Claudia Haarmann, Markus Rapedius, Ilka Rinke-Weiß, Sonja Stölzle-Feix, Niels Fertig, Søren Friis, Alison Obergrussberger, Tom A. Goetze |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Flexibility (engineering) Patch-Clamp Techniques Drug discovery Computer science business.industry High-throughput screening Ion Channels High-Throughput Screening Assays 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Automated patch clamp Drug Design Drug Discovery Animals Humans Patch clamp business 030217 neurology & neurosurgery Ion channel Computer hardware |
| Zdroj: | Expert opinion on drug discovery. 13(3) |
| ISSN: | 1746-045X |
| Popis: | Automated patch clamp (APC) devices have become commonplace in many industrial and academic labs. Their ease-of-use and flexibility have ensured that users can perform routine screening experiments and complex kinetic experiments on the same device without the need for months of training and experience. APC devices are being developed to increase throughput and flexibility. Areas covered: Experimental options such as temperature control, internal solution exchange and current clamp have been available on some APC devices for some time, and are being introduced on other devices. A comprehensive review of the literature pertaining to these features for the Patchliner, QPatch and Qube and data for these features for the SyncroPatch 384/768PE, is given. In addition, novel features such as dynamic clamp on the Patchliner and light stimulation of action potentials using channelrhodosin-2 is discussed. Expert opinion: APC devices will continue to play an important role in drug discovery. The instruments will be continually developed to meet the needs of HTS laboratories and for basic research. The use of stem cells and recordings in current clamp mode will increase, as will the development of complex add-ons such as dynamic clamp and optical stimulation on high throughput devices. |
| Databáze: | OpenAIRE |
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