Antimicrobial activity of Mimosa caesalpiniifolia Benth and its interaction with antibiotics against Staphylococcus aureus strains overexpressing efflux pump genes
Autor: | Glenn W. Kaatz, J.S. Lima Neto, Josiel Lobato Ferreira, Bruno Quirino Araújo, Humberto Medeiros Barreto, S.W.C. Silva, Daniel Dias Rufino Arcanjo, J.P. de Siqueira Júnior, Nayana Bruna Nery Monção, Antônia Maria das Graças Lopes Citó |
---|---|
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
Staphylococcus aureus Mimosa medicine.drug_class Tetracycline Antibiotics Microbial Sensitivity Tests medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology Antiporters Microbiology 03 medical and health sciences Bacterial Proteins Ciprofloxacin Staphylococcus epidermidis Ethidium 010608 biotechnology Candida albicans medicine Humans Norfloxacin Methylene Chloride 0303 health sciences biology 030306 microbiology Chemistry Tetracycline Resistance Staphylococcal Infections Antimicrobial biology.organism_classification Drug Resistance Multiple Anti-Bacterial Agents Plant Bark Efflux Multidrug Resistance-Associated Proteins Fluoroquinolones medicine.drug |
Zdroj: | Letters in Applied Microbiology. |
ISSN: | 1472-765X 0266-8254 |
Popis: | This study aimed to evaluate the antimicrobial activity of the dichloromethane fraction (DCMF) from the stem bark of Mimosa caesalpiniifolia and its effect on the activity of conventional antibiotics against Staphylococcus aureus strains overexpressing specific efflux pump genes. DCMF showed activity against S. aureus, Staphylococcus epidermidis and Candida albicans. Addition of DCMF at subinhibitory concentrations to the growth media enhanced the activity of norfloxacin, ciprofloxacin and ethidium bromide against S. aureus strains overexpressing norA suggesting the presence of efflux pump inhibitors in its composition. Similar results were verified for tetracycline against S. aureus overexpressing tetK, as well as, for ethidium bromide against S. aureus overexpressing qacC. These results indicate that M. caesalpiniifolia is a source of molecules able to modulate the fluoroquinolone- and tetracycline-resistance in S. aureus probably by inhibition of NorA, TetK and QacC respectively. SIGNIFICANCE AND IMPACT OF THE STUDY: Drug resistance is a common problem in patients with infectious diseases. Dichloromethane fraction from the stem bark of Mimosa caesalpiniifolia showed antimicrobial activity against Gram-positive bacterium Staphylococcus aureus and against Candida albicans, but did not show activity against Gram-negative specie Escherichia coli. Moreover, this fraction was able to potentiate the action of norfloxacin, ciprofloxacin and tetracycline against S. aureus strains overexpressing different efflux pump genes. Thus, Mimosa caesalpiniifolia is a source of efflux pump inhibitors which could be used in combination with fluoroquinolones or tetracycline in the treatment of infectious diseases caused by S. aureus strains overexpressing efflux pump genes. |
Databáze: | OpenAIRE |
Externí odkaz: |