The influence of astragalus polysaccharide and β-elemene on LX-2 cell growth, apoptosis and activation
| Autor: | Cheng-hua Li, Yi Liu, Hui Zhang, Shao-dan Li, Jin Zheng, Qinyou Ren, Yi Zhang, Yong-qi Dou, Lin Li, Ming-hui Yang, Heng-jun Shi, Li-tian Ma |
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| Rok vydání: | 2014 |
| Předmět: |
Liver Cirrhosis
Cell Survival 1.1 Normal biological development and functioning Clinical Sciences Apoptosis Cell Line Transforming Growth Factor beta1 Annexin Underpinning research Polysaccharides Hepatic stellate cells Hepatic Stellate Cells Medicine 2.1 Biological and endogenous factors Humans MTT assay Viability assay Aetiology Antigens CD44 Cell Proliferation biology Gastroenterology & Hepatology business.industry Cell growth β-elemene Liver Disease Gastroenterology General Medicine Astragalus Plant Molecular biology Actins Up-Regulation Astragalus polysaccharide Hyaluronan Receptors Cell culture Immunology biology.protein Hepatic stellate cell Public Health and Health Services business Digestive Diseases Sesquiterpenes Research Article |
| Zdroj: | BMC Gastroenterology BMC gastroenterology, vol 14, iss 1 |
| ISSN: | 1471-230X |
| Popis: | Background Activated hepatic stellate cells are the main source of excessive collagen deposition in liver fibrosis. Here we report the inhibitory effects of the combinational treatment of two natural products, astragalus polysaccharide (APS) and β-elemene (ELE) on the activation of human liver hepatic stellate cell line LX-2 cells. Methods Cultured LX-2 cells were treated with different concentrations of APS or ELE for 24 or 48 hours. Cell viability/apoptosis was measured by MTT assay and Annexin V/PI staining , activation related genes including α-SMA and CD44 expressions were measured by real-time PCR and western blot respectively. Results The majority of LX-2 cells showed morphological change in the presence of APS or ELE for 24 hours. Treatment with APS + ELE for 24 or 48 hours significantly inhabited the cell proliferation compared with APS or ELE treatment alone on LX-2 cells. APS + ELE may block the up-regulation of α-SMA and CD44 both in mRNA and protein levels through TGF-β pathway in LX-2 cells. Conclusion APS or ELE treatment alone on LX-2 cells could inhibit cell proliferation and induce apoptosis. The combinational treatment using APS + ELE significantly increased the killing efficiency on LX-2 cells. α-SMA and CD44 expressions was inhibited upon APS + ELE treatment through TGF-β pathway in LX-2 cells. The results indicated a novel treatment using natural products for liver diseases with anti-fibrotic effect. |
| Databáze: | OpenAIRE |
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