A primate-specific short GluN2A-NMDA receptor isoform is expressed in the human brain
| Autor: | Girish Vajramani, Chrysia-Maria Pegasiou, Steven D. Houghton, Ksenia Kurbatskaya, Mariana Vargas-Caballero, Xavier Altafaj, Diederik Bulters, Aminul I. Ahmed, Paul Grundy, Hanna Kariis, Hannah Warming, Aleksandra P. Pitera, Katrin Deinhardt |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Receptors de neurotransmissors lcsh:RC346-429 Neurotransmitter receptors Mice 0302 clinical medicine Postsynaptic potential Protein Isoforms Glutamate receptor Brain Human brain Middle Aged medicine.anatomical_structure NMDA receptor Female Human Gene isoform Adult Primates Protein subunit Short Report Neurosurgery Biology Receptors N-Methyl-D-Aspartate 03 medical and health sciences Cellular and Molecular Neuroscience Glutamatergic Young Adult Species Specificity medicine Animals Humans Resected Molecular Biology PSD-95 lcsh:Neurology. Diseases of the nervous system Aged Base Sequence Primate Associative learning 030104 developmental biology HEK293 Cells nervous system Synapses Primats Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Molecular Brain Molecular Brain, Vol 12, Iss 1, Pp 1-8 (2019) |
| Popis: | Glutamate receptors of the N-methyl-D-aspartate (NMDA) family are coincident detectors of pre- and postsynaptic activity, allowing Ca2+ influx into neurons. These properties are central to neurological disease mechanisms and are proposed to be the basis of associative learning and memory. In addition to the well-characterised canonical GluN2A NMDAR isoform, large-scale open reading frames in human tissues had suggested the expression of a primate-specific short GluN2A isoform referred to as GluN2A-S. Here, we confirm the expression of both GluN2A transcripts in human and primate but not rodent brain tissue, and show that they are translated to two corresponding GluN2A proteins present in human brain. Furthermore, we demonstrate that recombinant GluN2A-S co-assembles with the obligatory NMDAR subunit GluN1 to form functional NMDA receptors. These findings suggest a more complex NMDAR repertoire in human brain than previously thought. |
| Databáze: | OpenAIRE |
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