Decreased expression of UK114 is related to the differentiation status of human hepatocellular carcinoma
| Autor: | Hsin Ying Chou, Ya Ling Chen, Jin-Yuh Shew, Shiu Feng Huang, Chungming Chang, Yue Lin Tsai, Mong Liang Chen, Chiung-Tong Chen, Cheng Po Hu, Chin Liew Chong, Gar Yang Chau |
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| Rok vydání: | 2008 |
| Předmět: |
Pathology
medicine.medical_specialty Carcinoma Hepatocellular Tumor suppressor gene Epidemiology Cellular differentiation Blotting Western Down-Regulation Kidney Immunoenzyme Techniques Ribonucleases Western blot Cell Line Tumor medicine Biomarkers Tumor Humans Tissue Distribution Heat-Shock Proteins Messenger RNA Chi-Square Distribution medicine.diagnostic_test business.industry Liver Neoplasms Cancer medicine.disease Blotting Northern digestive system diseases Cell Transformation Neoplastic Oncology Liver Hepatocellular carcinoma Immunohistochemistry Liver cancer business |
| Zdroj: | Cancer epidemiology, biomarkersprevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 17(3) |
| ISSN: | 1055-9965 |
| Popis: | Previous studies have identified that the expression of UK114 is tissue specific and the protein has been found to be most abundant in liver and kidney. However, the expression of UK114 in human hepatocellular carcinoma and its relationship to differentiation and transformation of hepatocellular carcinoma have not been studied. In this study, the expression of UK114 in human hepatocellular carcinoma was examined by Northern and Western blot analyses. We found that UK114 was significantly down-regulated in most of hepatocellular carcinoma tissues compared with adjacent nontumor tissues (72.7%) at both mRNA and protein levels. We looked into the possibility that this decreased expression of UK114 in the hepatocellular carcinoma tissues may play a role in the differentiation or tumorigenicity of hepatocellular carcinoma. Immunohistochemical staining showed that the reduced expression of UK114 in hepatocellular carcinoma tissues was correlated with the tumor differentiation status as graded by the Edmondson-Steiner classification. On the other hand, overexpression of UK114 was not able to suppress the proliferation of human hepatoma cells and tumorigenicity in nude mice. These results suggest that UK114 does not seem to act as a tumor suppressor gene; however, it may useful as a biomarker that will assist in the grading of the differentiation status of hepatocellular carcinoma samples. (Cancer Epidemiol Biomarkers Prev 2008;17(3):535–42) |
| Databáze: | OpenAIRE |
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