Endophytic Akanthomyces sp. LN303 from Edelweiss Produces Emestrin and Two New 2-Hydroxy-4 Pyridone Alkaloids
| Autor: | Sergey B. Zotchev, Judith Wackerlig, Martina Oberhofer, Ernst Urban, Jia Jian Cao, Havva Büyük, Alexander Prado-Roller, Martin Zehl |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ions
biology Stereochemistry medicine.drug_class General Chemical Engineering Antibiotics Fungi General Chemistry EDELWEISS Antimicrobial agents Leontopodium nivale biology.organism_classification Plant use of endophytic fungi in defense Chemistry chemistry.chemical_compound Akanthomyces chemistry Organic compounds medicine QD1-999 4-Pyridone Nuclear magnetic resonance spectroscopy Bacteria Cordycipitaceae |
| Zdroj: | ACS Omega, Vol 6, Iss 3, Pp 2184-2191 (2021) |
| ISSN: | 2470-1343 |
| Popis: | In the search for new antibiotics, several fungal endophytes were isolated from the medicinal plant Leontopodium nivale subsp. alpinum (Edelweiss). The extract from one of these fungi classified as Akanthomyces sp. displayed broad-spectrum antibiotic activity against gram-negative bacteria and fungi. Further investigation into the composition of this extract using bioactivity-guided fractionation, HRMS, and nuclear magnetic resonance revealed two new 4-hydroxy-2-pyridone alkaloids (1, 2) and emestrin (3), an epidithiodioxopiperazine not previously known to be produced by a member of Cordycipitaceae. Further testing of purified compounds 1 and 2 proved that they are devoid of antibiotic activity, and all the activities observed in the crude extract could be assigned to emestrin (3), whose configuration was confirmed by crystallographic data. This study demonstrates, for the first time, that endophytic fungi from Edelweiss can produce new compounds, prompting further investigation into them for drug discovery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|