Retinal differentiation in an altricial bird species, Taeniopygia guttata: An immunohistochemical study
Autor: | Guadalupe Álvarez-Hernán, José Antonio de Mera-Rodríguez, Gervasio Martín-Partido, Javier Francisco-Morcillo, Alfonso Marzal, Joaquín Rodríguez-León, Eva M. Rico-Leo, Ismael Hernández-Núñez |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
animal structures Neurogenesis Blotting Western Morphogenesis Embryonic Development Chick Embryo Retina 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Neuroblast medicine Animals Eye Proteins Zebra finch Cell Proliferation biology Cell Differentiation biology.organism_classification Immunohistochemistry Sensory Systems Cell biology Ophthalmology Altricial 030104 developmental biology medicine.anatomical_structure Animals Newborn 030221 ophthalmology & optometry sense organs Precocial Finches Taeniopygia Biomarkers |
Zdroj: | Experimental eye research. 190 |
ISSN: | 1096-0007 |
Popis: | The bird retina offers an excellent model to investigate the mechanisms that coordinate the morphogenesis, histogenesis, and differentiation of neuron and glial cells. Although these developmental features have been intensively studied in the chicken (Gallus gallus, Linnaeus 1758), a precocial bird species, little is known about retinogenesis in altricial birds. The purpose of this study was to examine the differentiation of retinal cells in the altricial zebra finch (Taeniopygia guttata, Vieillot, 1817) and compare the results with those from previous studies in G. gallus. By using immunohistochemical techniques, the first differentiated TUJ1-/Isl1-positive neuroblasts were detected in the vitreal surface of the neuroblastic layer at later incubation times in T. guttata than in G. gallus (108 h vs 55 h). The immunoreactivity of these early differentiation markers coincided temporo-spatially with the appearance of the first PCNA-negative nuclei. Furthermore, the first visinin-positive photoreceptors (132 h vs 120 h) and the first Prox-1-immunoreactive neuroblasts (embryonic day 7.25 (E7.25) vs E6.5) were also detected at later embryonic stages in the retina of T. guttata than in the retina of G. gallus. At E13, one day before hatching, abundant PCNA- and pHisH3-immunoreactivities were detected in the T. guttata retina, while proliferation was almost absent in the G. gallus retina at perinatal stages. Therefore, these results suggest that cell differentiation in the retina is delayed in the altricial bird compared to precocial birds. Furthermore, the T. guttata retina was not completely developed at hatching, and abundant mitotically active precursor cells of retinal neurons were found, suggesting that retinal neurogenesis was intense at perinatal stages. |
Databáze: | OpenAIRE |
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