Dendritic cell membrane CD83 enhances immune responses by boosting intracellular calcium release in T lymphocytes
| Autor: | Mariana Pereira Pinho, Isabella Katz Migliori, José Alexandre Marzagão Barbuto, Elizabeth Alexandra Flatow |
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| Rok vydání: | 2014 |
| Předmět: |
T-Lymphocytes
T cell Immunology Immunoglobulins chemistry.chemical_element chemical and pharmacologic phenomena Calcium Biology Antibodies Calcium in biology Flow cytometry Immune system Antigens CD medicine Humans Immunology and Allergy Calcium Signaling LINFÓCITOS T Calcium signaling Immunity Cellular Membrane Glycoproteins medicine.diagnostic_test Cell Membrane hemic and immune systems Dendritic Cells Cell Biology Dendritic cell Cell biology medicine.anatomical_structure chemistry Gene Knockdown Techniques Tumor necrosis factor alpha |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | CD83 is a marker of mDCs directly related to their lymphostimulatory ability. Some data suggest that it has a central role in the immune system regulation, but how this function is performed remains to be determined. This work aimed to analyze the influence of CD83, present in mDCs, in the modulation of calcium signaling in T lymphocytes. Mo were differentiated into iDCs and activated with TNF-α. iDCs were treated, 4 h before activation, with siRNACD83, to reduce CD83 expression. Purified allogeneic T lymphocytes were labeled with the calcium indicator Fluo-4-AM, and calcium mobilization in the presence of mDCs was analyzed. CD83 knockdown mDCs induced lower calcium signal amplitude in T lymphocytes (29.0±10.0) compared with siRNAscr-treated mDCs (45.5±5.3). In another set of experiments, surface mDC CD83 was blocked with a specific mAb, and again, decreased calcium signaling in T lymphocytes was detected by flow cytometry and microscopy (fluorescence and confocal). In the presence of antibody, the percentage of responding T cells was reduced from 58.14% to 34.29%. As expected, anti-CD83 antibodies also reduced the proliferation of T lymphocytes (as assessed by CFSE dilution). Finally, in the absence of extracellular calcium, CD83 antibodies abrogated T cell signaling induced by allogeneic mDCs, suggesting that the presence of CD83 in mDC membranes enhances T lymphocyte proliferation by boosting calcium release from intracellular stores in these cells. |
| Databáze: | OpenAIRE |
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