Macluraxanthone B inhibits LPS-induced inflammatory responses in RAW264.7 and BV2 cells by regulating the NF-κB and MAPK signaling pathways
| Autor: | Hyuncheol Oh, Linsha Dong, Chi-Su Yoon, Dong-Sung Lee, Zhiming Liu, Youn-Chul Kim, Wonmin Ko, Sam Cheol Kim, Kwan-Woo Kim, Hwan Lee |
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| Rok vydání: | 2021 |
| Předmět: |
MAPK/ERK pathway
Lipopolysaccharides MAP Kinase Signaling System p38 mitogen-activated protein kinases Xanthones Immunology Anti-Inflammatory Agents Nitric Oxide Synthase Type II Toxicology Nitric Oxide Nitric oxide chemistry.chemical_compound Mice Immunology and Allergy Animals Pharmacology biology Kinase NF-kappa B General Medicine Molecular biology Heme oxygenase RAW 264.7 Cells chemistry Cyclooxygenase 2 Mitogen-activated protein kinase biology.protein Tumor necrosis factor alpha Signal transduction Signal Transduction |
| Zdroj: | Immunopharmacology and immunotoxicology. 44(1) |
| ISSN: | 1532-2513 |
| Popis: | Objective The prenylated xanthones compounds, macluraxanthone B (MCXB) was isolated from the MeOH extracts of Cudrania tricuspidata. In this study, we investigated the effect of MCXB on inflammatory response. Materials and methods Anti-inflammatory effects of MCXB were examined in lipopolysaccharide (LPS)-stimulated RAW264.7 and BV2 cells. We observed their anti-inflammatory effects by ELISA, western blot analysis, and immunofluorescence. Results MCXB significantly inhibited the LPS-stimulated production of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-6 (IL-6), and tumor necrosis factor (TNF)-α in RAW264.7 and BV2 cells. MCXB also reduced the LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 proteins. Incubating cells with MCXB prevented subsequent activation of the nuclear factor kappa B (NF-κB) signaling pathway by inhibiting the nuclear localization and DNA-binding activity of the p65 subunit induced by LPS. MCXB inhibited the phosphorylation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and p38 mitogen-activated protein kinases (MAPKs) in RAW264.7 and BV2 cells. MCXB induced the expression of heme oxygenase (HO)-1 protein, and the inhibitory effect of MCXB on nitric oxide production was partially reversed by a selective HO-1 inhibitor. Discussion and conclusions Our results suggested that the anti-inflammatory effect of MCXB is partly regulated by HO-1 induction. In conclusion, MCXB could be a useful candidate for the development of therapeutic and preventive agents to treat inflammatory diseases. |
| Databáze: | OpenAIRE |
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