Heterogeneity of the astrocytic AMPA-receptor transcriptome

Autor: Raphael Menke, Andrea Mölders, Angela Koch, Nikolaj Klöcker
Rok vydání: 2018
Předmět:
0301 basic medicine
Patch-Clamp Techniques
Brain development
Green Fluorescent Proteins
Central nervous system
Mice
Transgenic
AMPA receptor
Gating
Biology
Hippocampal formation
Membrane Potentials
Transcriptome
Mice
Xenopus laevis
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Glial Fibrillary Acidic Protein
medicine
Animals
Receptors
AMPA
Antigens
SOXE Transcription Factors
musculoskeletal
neural
and ocular physiology
Calcium-Binding Proteins
Microfilament Proteins
Glutamate receptor
Brain
Gene Expression Regulation
Developmental
Oligodendrocyte Transcription Factor 2
Excitatory Amino Acid Transporter 1
Mice
Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Animals
Newborn
nervous system
Neurology
Astrocytes
Proteoglycans
Neuroscience
030217 neurology & neurosurgery
Ionotropic effect
Zdroj: Glia. 66:2604-2616
ISSN: 0894-1491
DOI: 10.1002/glia.23514
Popis: Astrocytes form the largest class of glial cells in the central nervous system. They serve plenty of diverse functions that range from supporting the formation and proper operation of synapses to controlling the blood-brain barrier. For many of them, the expression of ionotropic glutamate receptors of the AMPA subtype (AMPARs) in astrocytes is of key importance. AMPARs form as macromolecular protein complexes, whose composition of the pore-lining GluA subunits and of an extensive set of core and peripheral complex constituents defines both their trafficking and gating behavior. Although astrocytic AMPARs have been reported to exhibit heterogeneous properties, their molecular composition is largely unknown. In this study, we sought to quantify the astrocytic AMPAR transcriptome during brain development and with respect to selected brain regions. Whereas the early postnatal pattern of AMPAR mRNA expression showed minor variation over time, it did show significant heterogeneity in different brain regions. Cerebellar astrocytes express a combination of AMPAR complex constituents that is remarkably distinct from the one in neocortical or hippocampal astrocytes. Our study provides a workflow and a first reference for future investigations into the molecular and functional diversity of glial AMPARs.
Databáze: OpenAIRE
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