Hydrogen Peroxide Resistance ofAcetobacter pasteurianusNBRC3283 and Its Relationship to Acetic Acid Fermentation
| Autor: | Takayuki Kaga, Akiko Okamoto-Kainuma, Yasunori Ehata, Morio Ishikawa, Yukimichi Koizumi, Manami Ikeda, Takemasa Osono |
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| Rok vydání: | 2008 |
| Předmět: |
Molecular Sequence Data
Applied Microbiology and Biotechnology Biochemistry Acetobacteraceae Analytical Chemistry chemistry.chemical_compound Acetic acid Acetobacter Amino Acid Sequence Cloning Molecular Hydrogen peroxide Molecular Biology Acetic Acid Microbial Viability Ethanol Sequence Homology Amino Acid biology Organic Chemistry Hydrogen Peroxide General Medicine Metabolism Catalase biology.organism_classification DNA-Binding Proteins Repressor Proteins chemistry Fermentation Mutation biology.protein Sequence Alignment Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 72:2526-2534 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.80136 |
| Popis: | The bacterium Acetobacter pasteurianus can ferment acetic acid, a process that proceeds at the risk of oxidative stress. To understand the stress response, we investigated catalase and OxyR in A. pasteurianus NBRC3283. This strain expresses only a KatE homolog as catalase, which is monofunctional and growth dependent. Disruption of the oxyR gene increased KatE activity, but both the katE and oxyR mutant strains showed greater sensitivity to hydrogen peroxide as compared to the parental strain. These mutant strains showed growth similar to the parental strain in the ethanol oxidizing phase, but their growth was delayed when cultured in the presence of acetic acid and of glycerol and during the acetic acid peroxidation phase. The results suggest that A. pasteurianus cells show different oxidative stress responses between the metabolism via the membrane oxidizing pathway and that via the general aerobic pathway during acetic acid fermentation. |
| Databáze: | OpenAIRE |
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