Tumor-Promoting Ly-6G+ SiglecFhigh Cells Are Mature and Long-Lived Neutrophils
| Autor: | Rapolas Zilionis, Marius Messemaker, Jeremy Gungabeesoon, Marie Siwicki, Mikael J. Pittet, Genevieve M. Gerhard, Ralph Weissleder, Etienne Meylan, Steffen Rickelt, Yunkang Lin, Anna Kohl, Camilla Engblom, Christina Pfirschke, Allon M. Klein |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Lung Neoplasms Neutrophils Angiogenesis medicine.medical_treatment ddc:616.07 Inbred C57BL law.invention Mice 0302 clinical medicine law Antigens Ly neutrophils cancer myeloid cells lcsh:QH301-705.5 Lung ddc:616 Tumor biology Immunosuppression Integrin alpha M Adenocarcinoma of Lung/metabolism/pathology Lung/pathology Ly/metabolism Adenocarcinoma of Lung General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences Lung Neoplasms/metabolism/pathology Sialic Acid Binding Immunoglobulin-like Lectins/metabolism Report Cell Line Tumor medicine Animals Antigens Neutrophils/pathology Cell Proliferation Sialic Acid Binding Immunoglobulin-like Lectins Tumor Cell Invasion Cancer medicine.disease Mice Inbred C57BL 030104 developmental biology Murine lung lcsh:Biology (General) Myeloid cells biology.protein Cancer research Suppressor 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 32, Iss 12, Pp 108164-(2020) Cell Reports Cell Reports, Vol. 32, No 12 (2020) P. 108164 Cell reports, Cambridge, MA : Cell Press, 2020, vol. 32, no. 12, art. no.108164, p. [1-19] |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2020.108164 |
| Popis: | Myeloid cells co-expressing the markers CD11b, Ly-6G, and SiglecF can be found in large numbers in murine lung adenocarcinomas and accelerate cancer growth by fostering tumor cell invasion, angiogenesis, and immunosuppression; however, some of these cells’ fundamental features remain unexplored. Here, we show that tumor-infiltrating CD11b+ Ly-6G+ SiglecFhigh cells are bona fide mature neutrophils and therefore differ from other myeloid cells, including SiglecFhigh eosinophils, SiglecFhigh macrophages, and CD11b+ Ly-6G+ myeloid-derived suppressor cells. We further show that SiglecFhigh neutrophils gradually accumulate in growing tumors, where they can live for several days; this lifespan is in marked contrast to that of their SiglecFlow counterparts and neutrophils in general, which live for several hours only. Together, these findings reveal distinct attributes for tumor-promoting SiglecFhigh neutrophils and help explain their deleterious accumulation in the tumor bed. Graphical Abstract Highlights • SiglecFhigh CD11b+ Ly-6G+ cells are bona fide neutrophils • SiglecFhigh neutrophils continuously accumulate in tumor-bearing lungs and BAL fluids • SiglecFhigh neutrophils are mature and non-proliferating cells • SiglecFhigh neutrophils are long-lived cells contrary to their SiglecFlow counterparts Neutrophils are essential defenders during inflammation and modulators of key cancer-associated activities with pro- or antitumor functions. However, cell markers that describe different neutrophil states are insufficiently known. Pfirschke et al. show that SiglecFhigh CD11b+ Ly-6G+ cells define mature and long-lived tumor-promoting neutrophils, which are distinct from other myeloid cells. |
| Databáze: | OpenAIRE |
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