Ovalbumin-derived peptides activate retinoic acid signalling pathways and induce regulatory responses through toll-like receptor interactions
| Autor: | Rosina López-Fandiño, Daniel Lozano-Ojalvo, Leticia Pérez-Rodríguez, Mónica Martínez-Blanco, Elena Molina |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
T-Lymphocytes Retinoic acid Dendritic cells regulatory T cells Mice chemistry.chemical_compound 0302 clinical medicine RAR-related orphan receptor gamma retinoic acid Cells Cultured Toll like receptors Toll-like receptor Nutrition and Dietetics biology Hydrolysis Toll-Like Receptors NF-kappa B peptide immunotherapy FOXP3 hemic and immune systems Regulatory T cells Acquired immune system Cell biology Interleukin 10 Female lcsh:Nutrition. Foods and food supply Protein Binding Signal Transduction Ovalbumin Tretinoin lcsh:TX341-641 chemical and pharmacologic phenomena toll like receptors Aldehyde Dehydrogenase 1 Family Article Immunophenotyping 03 medical and health sciences Animals Humans dendritic cells Retinal Dehydrogenase Peptide immunotherapy Transforming growth factor beta Coculture Techniques Peptide Fragments 030104 developmental biology chemistry biology.protein Biomarkers 030215 immunology Food Science |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Nutrients Volume 12 Issue 3 Nutrients, Vol 12, Iss 3, p 831 (2020) |
| Popis: | This study investigates the potential of a hydrolysate of ovalbumin with pepsin (OP) to preclude Th2-type immunity by the enhancement of tolerogenic dendritic cells (DCs) and regulatory T (Treg) cells. Through Toll-like receptor (TLR) stimulation, OP enhances the retinoic acid pathway on DCs by means of the induction of aldehyde dehydrogenase enzymes and transforming growth factor beta (TGF-&beta ), and it confers upon DC the ability to upregulate interleukin 10 (IL-10) as well as other tolerance-promoting mediators downstream of TRL signalling, such as IL-27, IL-33, Notch ligands, OX40L, and the transcription factors IRF4 and IRF8. OP-conditioned DCs induce the expansion of Foxp3+ and Tr1 cells in co-culture with CD4+ T cells. Furthermore, OP directly conditions CD4+ T cells from naï ve mice, without the mediation of DCs, to express aldehyde dehydrogenase (ALDH) enzymes and, in the presence of the Th2 cytokine IL-4 and exogenous TGF-&beta it enhances Foxp3 expression. It is noteworthy that, on CD4+ T cells isolated from egg-allergic mice, OP significantly enriches the levels of Foxp3+ and Foxp3+ ROR&gamma t+ CD4+ T cells. In conclusion, we show that food peptides may work, analogously to microbial-driven signals, through TLRs, to promote a tolerogenic phenotype on cells of the innate and adaptive immune system, a property that is further enhanced in the context of a Th2 cytokine-rich environment. |
| Databáze: | OpenAIRE |
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