Simvastatin prodrug micelles target fracture and improve healing
Autor: | Aaron Daluiski, Anand Dusad, Hongjiang Yuan, Edward V. Fehringer, P. Edward Purdue, Yijia Zhang, Yen Hsun Chen, Steven R. Goldring, Ke Ren, Zhenshan Jia, Dong Wang, Fei Li |
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Rok vydání: | 2015 |
Předmět: |
Male
Simvastatin Pharmaceutical Science Bone healing Pharmacology Micelle Bone morphogenetic protein 2 Article Cell Line Polyethylene Glycols Mice medicine Animals Prodrugs Femur MC3T3 Micelles Chemistry Bone fracture Prodrug medicine.disease In vitro Osteotomy Drug Liberation Systemic administration Hydroxymethylglutaryl-CoA Reductase Inhibitors Femoral Fractures |
Zdroj: | Journal of Controlled Release. 200:23-34 |
ISSN: | 0168-3659 |
Popis: | Simvastatin (SIM), a widely used anti-lipidaemic drug, has been identified as a bone anabolic agent. Its poor water solubility and the lack of distribution to the skeleton, however, have limited its application in the treatment of bone metabolic diseases. In this study, an amphiphilic macromolecular prodrug of SIM was designed and synthesized to overcome these limitations. The polyethylene glycol (PEG)-based prodrug can spontaneously self-assemble to form micelles. The use of SIM trimer as the prodrug’s hydrophobic segment allows easy encapsulation of additional free SIM. The in vitro studies showed that SIM/SIM-mPEG micelles were internalized by MC3T3 cells via lysosomal trafficking and consistently induced expression of both BMP2 and DKK1 mRNA, suggesting that the prodrug micelle retains the biological functions of SIM. After systemic administration, optical imaging suggests that the micelles would passively target to bone fracture sites associated with hematoma and inflammation. Furthermore, flow cytometry study revealed that SIM/SIM-mPEG micelles had preferred cellular uptake by inflammatory and resident cells within the fracture callus tissue. The treatment study using a mouse osteotomy model validated the micelles’ therapeutic efficacy in promoting bone fracture healing as demonstrated by micro-CT and histological analyses. Collectively, these data suggest that the macromolecular prodrug-based micelle formulation of SIM may have great potential for clinical management of impaired fracture healing. |
Databáze: | OpenAIRE |
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