Optimisation of in vitro culture conditions in B6CBF1 mouse embryos
| Autor: | Simon K. Walker, Bi-ke Zhu, Simon Maddocks |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Embryology Ratón Gonadotropins Equine Zygote media_common.quotation_subject Medicine (miscellaneous) Embryonic Development Cell Count Superovulation Biology Chorionic Gonadotropin Andrology Embryo Culture Techniques Polar body Mice medicine Animals Mineral oil media_common Embryogenesis Embryo In vitro Culture Media Blastocyst Reproductive Medicine Paraffin Immunology Animal Science and Zoology Female Reproduction Oils Developmental Biology Food Science medicine.drug |
| Zdroj: | Reproduction, nutrition, development. 44(3) |
| ISSN: | 0926-5287 |
| Popis: | This study was undertaken to investigate the effects of three media, volume and type of oil and frequency of observation on the in vitro development of mouse zygotes. B6CBF1 female mice (4 to 6 wk old) were superovulated using PMSG/hCG and mated with a proven fertile male of the same strain. Putative zygotes with polar bodies were collected from the oviducts of mated mice, 25-28 h after hCG injection, and were cultured in vitro. Embryo development was evaluated at either 96 h and 120 h or every 24 h for 120 h. The results obtained showed that the CZB medium was better than the KSOM and HCO3HTF media, and the use of 1 mL of paraffin oil was better than the use of 0.5 mL of paraffin oil. The effect of paraffin oil and mineral oil on embryo development was examined and the results indicated that the use of paraffin oil was better than the use of mineral oil. Repeated observations did not influence the proportion of embryos developing to blastocysts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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