Novel application of a Radial Water Tread maze can distinguish cognitive deficits in mice with traumatic brain injury
| Autor: | Satoshi Minoshima, Donna J. Cross, Gregory G. Garwin, Lisa Hysa, Dalia Murra, Josh C. Yumul, Marcella M. Cline, Warren C. Ladiges, David G. Cook |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Traumatic brain injury Water maze Motor Activity Neuropsychological Tests Article 03 medical and health sciences 0302 clinical medicine Physical medicine and rehabilitation Brain Injuries Traumatic medicine Memory impairment Animals Maze Learning Molecular Biology Gait Thigmotaxis Behavior Animal General Neuroscience Cognition medicine.disease Cognitive test Mice Inbred C57BL Disease Models Animal 030104 developmental biology Gait analysis Neurology (clinical) Psychology Cognition Disorders Neuroscience 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain research. 1657 |
| ISSN: | 1872-6240 |
| Popis: | Introduction The use of forced-swim, rat-validated cognition tests in mouse models of traumatic brain injury (TBI) raises methodological concerns; such models are vulnerable to a number of confounding factors including impaired motor function and stress-induced non-compliance (failure to swim). This study evaluated the ability of a Radial Water Tread (RWT) maze, designed specifically for mice, that requires no swimming to distinguish mice with controlled cortical impact (CCI) induced TBI and Sham controls. Methods Ten-week-old, male C57BL6/J mice were randomly assigned to receive either Sham (n = 14) or CCI surgeries (n = 15). Mice were tested for sensorimotor deficits via Gridwalk test and Noldus CatWalk gait analysis at 1 and 32 days post-injury. Mice received RWT testing at either 11 days (early time point) or 35 days (late time point) post-injury. Results Compared to Sham-treated animals, CCI-induced TBI resulted in significant impairment in RWT maze performance. Additionally, CCI injured mice displayed significant deficits on the Gridwalk test at both 1 day and 32 days post-injury, and impairment in the CatWalk task at 1 day, but not 32 days, compared to Shams. Conclusions The Radial Water Tread maze capitalizes on the natural tendency of mice to avoid open areas in favor of hugging the edges of an apparatus (thigmotaxis), and replaces a forced-swim model with water shallow enough that the animal is not required to swim, but aversive enough to motivate escape. Our findings indicate the RWT task is a sensitive species-appropriate behavioral test for evaluating spatial memory impairment in a mouse model of TBI. |
| Databáze: | OpenAIRE |
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