Comparison of kava (Piper methysticum Forst.) varieties by UV absorbance of acetonic extracts and high-performance thin-layer chromatography

Autor: Vincent Lebot, Laurent Legendre
Přispěvatelé: Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Identification
[SDV]Life Sciences [q-bio]
Composition des aliments
01 natural sciences
High-performance liquid chromatography
F30 - Génétique et amélioration des plantes
chemistry.chemical_compound
Chalcone
High performance thin layer chromatography
Rayonnement ultraviolet
Chemistry
Colorimétrie
Food composition kavalactones
Consommation alimentaire
04 agricultural and veterinary sciences
Composition chimique
Lactone
040401 food science
Flavokavins
Commercialisation
Qualité
medicine.drug
Method validation
F60 - Physiologie et biochimie végétale
Piper methysticum
E70 - Commerce
commercialisation et distribution
Biodiversity and horticulture
Kava varieties
Absorbance
0404 agricultural biotechnology
Botany
medicine
Variété
Kavain
Q04 - Composition des produits alimentaires
Technique analytique
Kava
Chromatography
Chromatographie en couche mince
Food analysis
010401 analytical chemistry
Colorimeter
Flavonoïde
Composition globale
HPTLC fingerprinting
Kavalactone
Roots
0104 chemical sciences
Collection de matériel génétique
Légume racine
HPLC
Food Science
Zdroj: Journal of Food Composition and Analysis
Journal of Food Composition and Analysis, Elsevier, 2016, 48, pp.25-33. ⟨10.1016/j.jfca.2016.01.009⟩
ISSN: 0889-1575
1096-0481
Popis: The development of quality standards to regulate the trade of kava (Piper methysticum) in the Pacific necessitates new analytical techniques for routine control of high-quality raw material. In the present study, varieties and parts of the plant were compared for their kavalactone and flavokavin (FK) profiles. Three hundred and eight samples corresponding to 25 noble, 25 two-days and five wichmannii varieties were collected from 203 different plants. Commercial samples were also analysed to test the technique. Overall, 353 samples were prepared (221 roots, 114 stumps, nine basal stems and nine stem peelings). Their acetonic extracts were measured for UV absorbance and analysed by HP-TLC. Two ratios were computed after scanning the plates at 245 nm (K/KL = kavain/total kavalactones) and 366 nm (FK/KL = flavokavins/kavalactones). The results indicate that noble varieties suitable for daily consumption of kava are characterised by high K/KL and low FK/KL. At 400 nm, the mean UV absorbance of acetonic extracts from noble, two-days and wichmannii varieties are 0.69, 0.94 and 1.04, respectively. The significant (+0.757**) correlation between the total FK content and the extracts absorbance at 400 nm indicates that a simple colorimeter can be used to detect poor-quality raw material. (Resume d'auteur)
Databáze: OpenAIRE
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