Serum Free Light Chain Assay: Shift Toward a Higher κ/λ Ratio
| Autor: | Christof Schild, Vera Ulrike Bacher, Martin Andres, Thomas Pabst, Alexander Benedikt Leichtle, Barbara Rindlisbacher, Nazanin Sédille-Mostafaie, Florence Egger |
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| Rok vydání: | 2019 |
| Předmět: |
Immunofixation
030213 general clinical medicine Paraproteinemias Renal function Immunoglobulin light chain 03 medical and health sciences Immunoglobulin kappa-Chains 0302 clinical medicine Immunoglobulin lambda-Chains Serum free Nephelometry and Turbidimetry medicine Humans Renal Insufficiency Diagnostic Errors biology Chemistry Reproducibility of Results General Medicine Gel electrophoresis of proteins Blood Protein Electrophoresis Molecular biology Monoclonal gammopathy C-Reactive Protein 030220 oncology & carcinogenesis Monoclonal biology.protein medicine.symptom Nephelometry Glomerular Filtration Rate |
| Zdroj: | The journal of applied laboratory medicine. 5(1) |
| ISSN: | 2576-9456 |
| Popis: | BackgroundThe analysis of serum free light chains (FLCs) is clinically relevant for the diagnosis and therapeutic management of clonal plasma cell disorders. This study compares the performance of monoclonal and polyclonal FLC κ and λ assays in clinical samples determined in a single academic center.MethodsSerum FLCs were analyzed from 102 patients using the Freelite (Binding Site) and N Latex (Siemens) assays on the BN ProSpec System (Siemens). When available, data for protein electrophoresis, immunofixation, C-reactive protein, and estimated glomerular filtration rate (eGFR) were combined with FLC results to evaluate performance.ResultsMethod evaluation showed acceptable imprecision and inaccuracy measures of ConclusionsUnlike the Freelite assay, κ/λ ratios analyzed with the N Latex assay were not affected by renal failure. Both methods showed acceptable performances using nephelometry, but they were poorly correlated. A shift toward κ/λ ratios might impair the specificity of borderline increased κ/λ results. This should be considered when interpreting FLC κ and λ results. |
| Databáze: | OpenAIRE |
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