Isolation and analysis of mouse microglial cells
| Autor: | Jenny A. Garcia, Astrid E. Cardona, Sandra M. Cardona |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Central Nervous System
Male Immunology Central nervous system Cell Separation Biology Peripheral blood mononuclear cell Article Flow cytometry Mice Immune system Immunochemistry medicine Animals Homeostasis Cells Cultured Myeloid Progenitor Cells medicine.diagnostic_test Microglia Immunity Flow Cytometry Immunohistochemistry Cell biology Mice Inbred C57BL medicine.anatomical_structure Female Immunostaining |
| Zdroj: | Current Protocols in Immunology |
| ISSN: | 1934-368X |
| Popis: | Microglia are mononuclear phagocytes that make up about 10% of the central nervous system (CNS). They are known for their surveillant behavior which comprises continuously monitoring neural tissue by extending and retracting their processes. Microglial cells are derived from myeloid progenitor cells and play important roles in homeostasis, inflammatory and immune responses in the brain. This Unit describes several microglial cell isolation protocols (Basic Protocol 1, Alternate Protocol, and Basic Protocol 2) that can be easily adapted for projects requiring a rapid and efficient analysis of mouse microglial cells by flow cytometry (Support Protocol 1). Methods for visualizing microglial cells using in situ immunohistochemistry (Basic Protocol 3) and immunochemistry in free-floating sections (Basic Protocol 4) are also included. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text