A new U2 RNA secondary structure provided by phylogenetic analysis of trypanosomatid U2 RNAs
| Autor: | T Hartshorne, N Agabian |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
RNA Splicing
Trypanosoma cruzi Trans-splicing Molecular Sequence Data Restriction Mapping Trypanosoma brucei brucei Biology Nucleic acid secondary structure RNA Small Nuclear Consensus Sequence Genetics Animals Guide RNA Small nucleolar RNA Phylogeny Base Sequence RNA Non-coding RNA Long non-coding RNA Blotting Southern Gene Expression Regulation Genes Nucleic Acid Conformation Trypanosomatina Pseudoknot RNA Protozoan Developmental Biology |
| Zdroj: | Genesdevelopment. 4(12A) |
| ISSN: | 0890-9369 |
| Popis: | A new model of U2 RNA secondary structure has been developed by comparing U2 RNA sequences from distantly related trypanosomatids, which process their RNAs by trans-splicing, and U2 RNAs from cis-splicing organisms. The trypanosomatid U2 RNA retains structural similarity in important functional domains of cis-splicing U2 RNAs yet differs from previous consensus models in that only two helices, rather than three, can form in the stem-loop II region. This alteration eliminates the capacity for pseudoknot formation and produces a single-stranded region 3' to stem-loop II, which may be accessible for snRNP protein binding. Trypanosomatid U2 RNAs differ in the putative branchpoint recognition sequences, which completely diverge from the conserved GUAGUA consensus of cis-splicing organisms. |
| Databáze: | OpenAIRE |
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