Interleukin-4 inhibits spontaneous and parathyroid hormone-related protein-stimulated osteoclast formation in mice
| Autor: | Sumiya Eto, Kenichi Watanabe, Yoichiro Nakano, Keizo Kasono, Yosuke Okada, Kazumi Ura, Kanji Sato, Toshitaka Nakamura, Isao Morimoto |
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| Rok vydání: | 2009 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Esophageal Neoplasms Ratón Endocrinology Diabetes and Metabolism Mice Nude Osteoclasts chemistry.chemical_element Parathyroid hormone Cell Count Calcium Bone resorption Mice In vivo Osteoclast Internal medicine Tumor Cells Cultured medicine Animals Humans Orthopedics and Sports Medicine Femur Bone Resorption Interleukin 4 Mice Inbred BALB C Mice Inbred ICR Parathyroid hormone-related protein Chemistry Parathyroid Hormone-Related Protein Proteins Infusion Pumps Implantable Peptide Fragments Recombinant Proteins Disease Models Animal Endocrinology medicine.anatomical_structure Parathyroid Hormone Carcinoma Squamous Cell Hypercalcemia Female Interleukin-4 Neoplasm Transplantation |
| Zdroj: | Journal of Bone and Mineral Research. 9:1533-1539 |
| ISSN: | 0884-0431 |
| Popis: | We examined the in vivo effects of recombinant murine IL-4 (rmIL-4) on spontaneous and stimulated mouse osteoclast formation. EC-GI cells, which produce PThrP and IL-1 alpha, were explanted in nude mice. These EC-GI cell-bearing nude mice developed hypercalcemia (4.90 +/- 0.68 mM), and the calcium levels were decreased to near normal (3.48 +/- 0.73 mM, p < 0.05) at day 3 by continuous infusion of rmIL-4 at a dose of 7 micrograms/day. When infused with 0.6 nmol/day of PTHrP(1-34) in ICR mice, rmIL-4 at a dose of 1 or 5 micrograms/day for 3 days caused a marked inhibitory effect on hypercalcemia induced by PTHrP(1-34) (3.73 +/- 0.56-2.54 +/- 0.14 mM, p < 0.01). However, rmIL-4 alone did not change the serum calcium in mice. Histomorphometric analysis revealed that rmIL-4 inhibits both spontaneous and PTHrP(1-34)-stimulated osteoclast formation in mice, with a decrease in osteoclastic surface and in the number of osteoclasts per mm bone surface, respectively. We conclude that IL-4 inhibits spontaneous and stimulated bone resorption resulting from inhibition of osteoclast formation and modulates the development of humoral hypercalcemia of malignancy. |
| Databáze: | OpenAIRE |
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