Modulation of functional characteristics of resident and thioglycollate-elicited peritoneal murine macrophages by a recombinant banana lectin

Autor: Aleksandra Inic-Kanada, Radmila Djokic, Ana Filipovic, Dejana Kosanovic, Marija Gavrović-Jankulović, Marijana Stojanovic, Ivana Lukic, Emilija Marinkovic
Rok vydání: 2017
Předmět:
0301 basic medicine
Physiology
Lipopolysaccharide Receptors
lcsh:Medicine
Stimulation
Pathology and Laboratory Medicine
Biochemistry
Immune Receptors
Mice
White Blood Cells
0302 clinical medicine
Transforming Growth Factor beta
Animal Cells
Immune Physiology
Lectins
Medicine and Health Sciences
Enzyme-Linked Immunoassays
lcsh:Science
Receptor
Immune Response
Toll-like Receptors
Mice
Inbred BALB C
Innate Immune System
Immune System Proteins
Multidisciplinary
Chemistry
Interleukin-12
Recombinant Proteins
Interleukin-10
Arginase
Thioglycolates
030220 oncology & carcinogenesis
Cytokines
Tumor necrosis factor alpha
Plant Lectins
Cellular Types
Protein Binding
Signal Transduction
Research Article
Immune Cells
CD14
Immunology
Dose-Response Relationship
Immunologic
Nitric Oxide
Research and Analysis Methods
Proinflammatory cytokine
03 medical and health sciences
Signs and Symptoms
Diagnostic Medicine
Animals
Immunologic Factors
Secretion
Immunoassays
Cell Proliferation
Inflammation
Blood Cells
Tumor Necrosis Factor-alpha
Macrophages
lcsh:R
Biology and Life Sciences
Proteins
Cell Biology
Macrophage Activation
Molecular Development
Molecular biology
Toll-Like Receptor 2
Mice
Inbred C57BL
TLR2
030104 developmental biology
Gene Expression Regulation
Immune System
Macrophages
Peritoneal
Immunologic Techniques
lcsh:Q
Physiological Processes
Developmental Biology
Zdroj: PLoS ONE, Vol 12, Iss 2, p e0172469 (2017)
PLoS One
PLoS One / Public Library of Science
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0172469
Popis: We demonstrated that a recombinant banana lectin (rBanLec), which structural characteristics and physiological impacts highly resemble those reported for its natural counterparts, binds murine peritoneal macrophages and specifically modulates their functional characteristics. By using rBanLec in concentrations ranging from 1 mu g to 10 mu g to stimulate resident (RMs) and thioglycollate-elicited (TGMs) peritoneal macrophages from BALB/c and C57BL/6 mice, we have shown that effects of rBanLec stimulation depend on its concentration but also on the functional status of macrophages and their genetic background. rBanLec, in a positive dose-dependent manner, promotes the proliferation of TGMs from both BALB/c and C57BL/6 mice, while its mitogenic influence on RMs is significantly lower (BALB/c mice) or not detectable (C57BL/6 mice). In all peritoneal macrophages, irrespective of their type and genetic background, rBanLec, in a positive dose dependent manner, enhances the secretion of IL-10. rBanLec stimulation of RMs from both BALB/c and C57BL/6 resulted in a positive dose-dependent promotion of proinflammatory phenotype (enhancement of NO production and IL-12 and TNF alpha secretion, reduction of arginase activity). Positive dose-dependent skewing toward proinflammatory phenotype was also observed in TGMs from C57BL/6 mice. However, the enhancement of rBanLec stimulation promotes skewing of TGMs from BALB/c mice towards anti-inflammatory profile (reduction of NO production and IL-12 secretion, enhancement of arginase activity and TGF alpha and IL-4 secretion). Moreover, we established that rBanLec binds oligosaccharide structures of TLR2 and CD14 and that blocking of signaling via these receptors significantly impairs the production of TNFa and NO in BALB/c macrophages. Since the outcome of rBanLec stimulation depends on rBanLec concentration as well as on the functional characteristics of its target cells and their genetic background, further studies are needed to investigate its effects under physiological and specific pathological conditions. Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3037]
Databáze: OpenAIRE
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