Induction of antibody forming cells with specificity for Candida albicans mannoproteins in cultures of human peripheral blood lymphocytes
| Autor: | M. Boccanera, Luciana Giordani, Alma L. Luzzati, Antonio Cassone, Elena Giacomini |
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| Rok vydání: | 1993 |
| Předmět: |
Antigens
Fungal Immunology Blood Donors Enzyme-Linked Immunosorbent Assay Cross Reactions Epitope Microbiology Fungal Proteins Epitopes Antigen Antibody Specificity Candida albicans Humans Immunology and Allergy Antibody-Producing Cells Antibodies Fungal chemistry.chemical_classification Membrane Glycoproteins biology ELISPOT biology.organism_classification In vitro Corpus albicans Immunoglobulin Isotypes chemistry biology.protein Antibody Glycoprotein |
| Zdroj: | Journal of Immunological Methods. 164:203-211 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(93)90313-v |
| Popis: | A method is described for the induction of a specific antibody response to Candida albicans in cultures of normal human peripheral blood lymphocytes (PBL). PBL were cultured in the presence of whole C. albicans cells and the antibody response was evaluated by the ELISPOT technique, on plastic wells coated with a purified candidal cell well mannoprotein (MP). Under the conditions described here, a specific antibody response was obtained in all of the eight donors tested. The response was antigen-dependent and antigen-specific, peaked around day 10–12 of culture and the antibodies belonged to both the IgM and the IgG isotypes. By testing the cultured cells on MP from different Candida species, the method per detection of antibodies directed against MP epitopes shared by C. albicans and C. parapsilosis. |
| Databáze: | OpenAIRE |
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