Evaluation of mutations in penicillin binding protein-3 gene of non-typeableHaemophilus influenzaeisolated from the nasopharynx of children with acute otitis media
| Autor: | Akihiro Sakai, Dewan S. Billal, Muneki Hotomi, Jun Shimada, Kazuma Yamauchi, Keiji Fujihara, Noboru Yamanaka, Shinji Tamura |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Haemophilus Infections Penicillin binding proteins medicine.drug_class Antibiotics Gene Expression medicine.disease_cause Polymerase Chain Reaction beta-Lactamases Haemophilus influenzae Microbiology Bacterial Proteins Nasopharynx Ampicillin medicine Humans Penicillin-Binding Proteins Point Mutation Child DNA Primers Antibacterial agent Mutation biology Point mutation Pasteurellaceae Infant General Medicine biology.organism_classification Virology Otitis Media Otorhinolaryngology Child Preschool Acute Disease Female medicine.drug |
| Zdroj: | Acta Oto-Laryngologica. 125:180-183 |
| ISSN: | 1651-2251 0001-6489 |
| DOI: | 10.1080/00016480410020301 |
| Popis: | Younger children tend to harbor more resistant strains because they are exposed to these pathogens more often through contacts with siblings or attendance at day-care centers and are frequently treated with antibiotics. The high prevalence of BLNAR strains should be taken into account in the treatment of AOM in young children.Non-beta-lactamase-producing ampicillin-resistant (BLNAR) strains with mutations in penicillin-binding protein (PBP) genes of Haemophilus influenzae have been prevalent recently among younger children.We investigated mutations in the ftsI gene encoding PBP-3 of H. influenzae isolated from the nasopharynx of children with acute otitis media (AOM) using polymerase chain reaction (PCR).Strains containing the bla gene (beta-lactamase-producing ampicillin-resistant) were identified in 4.7% of cases. Strains with mutations in the ftsI gene (BLNAR) were identified in 23.3% of cases. Strains without mutations in the ftsI gene and that did not contain the bla gene (non-beta-lactamase-producing ampicillin-susceptible) were identified in 70.7% of cases. Strains with both expression of the bla gene and mutations in the ftsI gene (beta-lactamase-producing amoxicillin clavulanate-resistant) were identified in 1.3% of cases. The MICs of ampicillin against the strains evaluated in this study were 0.5-2.0 microg/ml. Cefditoren-pivoxil had the lowest MIC90 against the strains (0.06 microg/ml). Strains with mutations in the ftsI gene (BLNAR) were broadly identified among young children. |
| Databáze: | OpenAIRE |
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