Interferon inhibition of the primary in vitro antibody response to a thymus-independent antigen
| Autor: | Samuel Baron, Howard M. Johnson, Joann A. Bukovic |
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| Rok vydání: | 1975 |
| Předmět: |
Hot Temperature
Time Factors Lipopolysaccharide Immunology Cell Mice Nude Hemolytic Plaque Technique Spleen Biology L Cells (Cell Line) Mice Tissue culture chemistry.chemical_compound L Cells Antigen Interferon Escherichia coli medicine Animals Antigens Bacterial B-Lymphocytes Macrophages Polysaccharides Bacterial Molecular biology In vitro Mice Inbred C57BL medicine.anatomical_structure chemistry Antibody Formation Female Interferons medicine.drug |
| Zdroj: | Cellular Immunology. 20:104-109 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/0008-8749(75)90089-1 |
| Popis: | Partially purified and crude mouse L cell interferon preparations inhibited the in vitro plaque-forming cell (PFC) response of mouse C57B1/6 spleen cells to the T-cell independent lipopolysaccharide antigen of Escherichia coli 0127. PFC responses of 5-day cultures were inhibited approximately 70–90% by 100–200 NIH reference units of interferon/culture. A similar inhibitory effect was obtained with spleen cells from athymic (nude) mice homozygous for the nu/nu allele. Spleen cultures depleted of adherent cells were also inhibited in their anti-0127 PFC response by interferon. Interferon, then, appears capable of inhibiting the PFC response to E. coli 0127 via direct action on B cells. Heating experiments along with the use of interferon preparations of different specific activities suggest that the inhibition was due to the interferon in the preparations. |
| Databáze: | OpenAIRE |
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