A 7-Gene Signature Depicts the Biochemical Profile of Early Prefibrotic Myelofibrosis
| Autor: | Mark Burton, Ole Weis Bjerrum, Rasmus Dahl-Sørensen, Inger Stamp, Henrik Bondo, Mats Ehinger, Lasse Kjær, Caroline Hasselbalch Riley, Thomas Stauffer Larsen, Vibe Skov, Mads Thomassen, Nana Brochmann, Morten K. Jensen, Karsten Bork Nielsen, Torben A Kruse, Jürgen Thiele, Ann Brinch Madelung, Hans Carl Hasselbalch |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
Pathology Physiology Biopsy Gene Expression lcsh:Medicine Genetic Networks Pathology and Laboratory Medicine White Blood Cells 0302 clinical medicine Animal Cells Bone Marrow Immune Physiology Medicine and Health Sciences Leukocytosis lcsh:Science Immune Response Aged 80 and over Multidisciplinary Hematology Genomics Middle Aged Body Fluids Blood medicine.anatomical_structure 030220 oncology & carcinogenesis Female Anatomy Cellular Types medicine.symptom Network Analysis Research Article Adult Computer and Information Sciences medicine.medical_specialty Anemia Immune Cells Immunology Surgical and Invasive Medical Procedures 03 medical and health sciences Signs and Symptoms Diagnostic Medicine Genetics medicine Journal Article Humans Gene Regulation Myelofibrosis Aged Inflammation Blood Cells Essential thrombocythemia business.industry Gene Expression Profiling lcsh:R Biology and Life Sciences Computational Biology Cell Biology Gene signature Genome Analysis medicine.disease Gene expression profiling Gene Expression Regulation Primary Myelofibrosis Immune System lcsh:Q Bone marrow Differential diagnosis business 030215 immunology |
| Zdroj: | PLoS ONE, Vol 11, Iss 8, p e0161570 (2016) Skov, V, Burton, M, Thomassen, M, Stauffer Larsen, T, Riley, C H, Brinch Madelung, A, Kjær, L, Bondo, H, Stamp, I, Ehinger, M, Dahl-Sørensen, R, Brochmann Mortensen, N, Nielsen, K, Thiele, J, Jensen, M K, Weis Bjerrum, O, Kruse, T A & Hasselbalch, H C 2016, ' A 7-Gene Signature Depicts the Biochemical Profile of Early Prefibrotic Myelofibrosis ', P L o S One, vol. 11, no. 8, e0161570, pp. 1-15 . https://doi.org/10.1371/journal.pone.0161570 Skov, V, Burton, M, Thomassen, M, Larsen, T S, Hasselbalch Riley, C, Brinch Madelung, A, Kjær, L, Bondo, H, Stamp, I, Ehinger, M, Dahl-Sørensen, R, Brochmann Mortensen, N, Nielsen, K, Thiele, J, Jensen, M K, Kruse, T A, Bjerrum, O W & Hasselbalch, H C 2015, ' A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis ', Haematologica, vol. 100, no. S1, E1315, pp. 526 . < http://www.haematologica.org/content/100/supplement_1/1.full-text.pdf+html > Skov, V, Burton, M, Thomassen, M, Stauffer Larsen, T, Riley, C H, Madelung, A B, Kjaer, L, Bondo, H, Stamp, I, Ehinger, M, Dahl-Sørensen, R, Brochmann, N, Nielsen, K, Thiele, J, Jensen, M K, Weis Bjerrum, O, Kruse, T A & Hasselbalch, H C 2016, ' A 7-Gene Signature Depicts the Biochemical Profile of Early Prefibrotic Myelofibrosis ', PLoS ONE, vol. 11, no. 8, e0161570 . https://doi.org/10.1371/journal.pone.0161570 PLoS ONE Skov, V, Burton, M, Thomassen, M, Larsen, T S, Hasselbalch Riley, C, Brinch Madelung, A, Kjær, L, Bondo, H, Stamp, I, Ehinger, M, Dahl-Sørensen, R, Brochmann Mortensen, N, Nielsen, K, Thiele, J, Jensen, M K, Bjerrum, O W, Kruse, T A & Hasselbalch, H C 2015, ' A 7-gene signature depicts the biochemical profile of early prefibrotic myelofibrosis ', 20th Congress of European Hematology Association (EHA), Vienna, Austria, 11/06/2015-14/06/2015 . < http://www.haematologica.org/content/100/supplement_1/1.full-text.pdf+html > |
| ISSN: | 1932-6203 |
| Popis: | Recent studies have shown that a large proportion of patients classified as essential thrombocythemia (ET) actually have early primary prefibrotic myelofibrosis (prePMF), which implies an inferior prognosis as compared to patients being diagnosed with so-called genuine or true ET. According to the World Health Organization (WHO) 2008 classification, bone marrow histology is a major component in the distinction between these disease entities. However, the differential diagnosis between them may be challenging and several studies have not been able to distinguish between them. Most lately, it has been argued that simple blood tests, including the leukocyte count and plasma lactate dehydrogenase (LDH) may be useful tools to separate genuine ET from prePMF, the latter disease entity more often being featured by anemia, leukocytosis and elevated LDH. Whole blood gene expression profiling was performed in 17 and 9 patients diagnosed with ET and PMF, respectively. Using elevated LDH obtained at the time of diagnosis as a marker of prePMF, a 7-gene signature was identified which correctly predicted the prePMF group with a sensitivity of 100% and a specificity of 89%. The 7 genes included MPO, CEACAM8, CRISP3, MS4A3, CEACAM6, HEMGN, and MMP8, which are genes known to be involved in inflammation, cell adhesion, differentiation and proliferation. Evaluation of bone marrow biopsies and the 7-gene signature showed a concordance rate of 71%, 79%, 62%, and 38%. Our 7-gene signature may be a useful tool to differentiate between genuine ET and prePMF but needs to be validated in a larger cohort of "ET" patients. |
| Databáze: | OpenAIRE |
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