Purification of Antibiotics from Physarum Gyrosum by High Pressure Liquid Chromatography
| Autor: | Richard L. Taylor, M. F. Mallette |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Chromatography
Aqueous normal-phase chromatography Elution Chemistry Ethyl acetate Biochemistry High-performance liquid chromatography Thin-layer chromatography Anti-Bacterial Agents Physarum chemistry.chemical_compound Paper chromatography Column chromatography Genetics Supercritical fluid chromatography Chromatography High Pressure Liquid |
| Zdroj: | Preparative Biochemistry. 8:241-257 |
| ISSN: | 0032-7484 |
| DOI: | 10.1080/00327487808065524 |
| Popis: | A family of five antibiotic substances was isolated from the slime mold Physarum gyrosurn by high pressure liquid chromatography (HPLC). For this purpose, mold was cultured for two weeks in a liquid medium. Soluble products were harvested by rotary evaporation of medium and extraction with 1-butanol. Paper chromatography in ethyl acetate :pyridine:water (2:2:1 v/v) was used for preliminary fractionation. Active components were separated by HPLC with a reverse-phase column packed with Bondapack C18/Porasil B (Waters Associates) and were eluted with a linear gradient of methanol:water increasing from 70 to 100% methanol over 90 minutes. Puri-fication was completed by rechromatographing individual fractions. Purity of the active components was verified by HPLC and thin layer chromatography. Activity assays against Bacillus cereus showed these materials to be bacteriostatic rather than bacteriocidal. |
| Databáze: | OpenAIRE |
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