Effect of selective inhibition or activation of PGE2 EP1 receptor on glomerulosclerosis
| Autor: | Zhi Qiu, Jun Yin, Jing Liu, Xu Chen, Xiaolan Chen, Yu-Yin Xu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Cancer Research 17-phenyl- trinor-prostaglandin E2 ethyl amide Apoptosis Kidney Biochemistry Nephrectomy TRPC1 Mice eIF-2 Kinase 0302 clinical medicine Glomerulonephritis transient receptor potential channel 1 glucose-regulated protein 78 Receptor Dibenz(b f)(1 4)oxazepine-10(11H)-carboxylic acid 8-chloro- 2-acetylhydrazide Endoplasmic Reticulum Chaperone BiP Cells Cultured Heat-Shock Proteins Chemistry Articles Receptor antagonist Endoplasmic Reticulum Stress Receptors Prostaglandin E EP1 Subtype medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Mesangial Cells Molecular Medicine lipids (amino acids peptides and proteins) glomerulosclerosis Agonist medicine.medical_specialty endocrine system Prostaglandin Antagonists medicine.drug_class Prostaglandin E2 receptor Dinoprostone Transforming Growth Factor beta1 03 medical and health sciences Downregulation and upregulation Internal medicine Genetics medicine Animals Molecular Biology TRPC Cation Channels SC-19220 Glomerulosclerosis medicine.disease prostaglandin E2 receptor 1 Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | Prostaglandin E2 (PGE2) is involved in numerous physiological and pathological processes of the kidney via its four receptors. A previous study has suggested that a defect in the PGE2 receptor 1 (EP1) gene markedly suppressed the transforming growth factor‑β1 (TGF‑β1)‑induced mesangial cell (MC) proliferation and extracellular matrix aggregation. Therefore, the present study aimed to adopt a pharmacological method of specifically suppressing or activating the EP1 receptor to further verify and demonstrate these results. The EP1 receptor antagonist SC‑19220 and EP1 receptor agonist 17‑phenyl‑trinor‑PGE2 ethyl amide (17‑pt‑PGE2) were selectively used to treat five‑sixths nephrectomy renal fibrosis model mice and TGF‑β1‑stimulated MCs. An Alpha screen PGE2 assay kit, flow cytometry, western blotting and immunohistochemical techniques were adopted to perform in vivo and in vitro experiments. The present results suggested that compared with the control group, the selective EP1 receptor antagonist SC‑19220 improved renal function, markedly reduced the plasma blood urea nitrogen and creatinine levels (P |
| Databáze: | OpenAIRE |
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