Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation
| Autor: | Baoyu Chen, Klaus Brinkmann, Klaus Ebnet, Tim Steinbacher, Anna Julia Squarr, Michael K. Rosen, Sven Bogdan |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Rotation Morphogenesis Arp2/3 complex macromolecular substances Article Extracellular matrix 03 medical and health sciences 0302 clinical medicine Cell Movement Animals Drosophila Proteins Cytoskeleton Actin Research Articles biology Correction Cell migration Cell Biology Actin cytoskeleton Cadherins Actins Cell biology Extracellular Matrix 030104 developmental biology Drosophila melanogaster Multiprotein Complexes biology.protein Filopodia 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | The atypical cadherin Fat2 binds the WAVE regulatory complex (WRC) and acts with receptor tyrosine phosphatase Dlar through the WRC to control collective cell migration during Drosophila oogenesis. Directional cell movements during morphogenesis require the coordinated interplay between membrane receptors and the actin cytoskeleton. The WAVE regulatory complex (WRC) is a conserved actin regulator. Here, we found that the atypical cadherin Fat2 recruits the WRC to basal membranes of tricellular contacts where a new type of planar-polarized whip-like actin protrusion is formed. Loss of either Fat2 function or its interaction with the WRC disrupts tricellular protrusions and results in the formation of nonpolarized filopodia. We provide further evidence for a molecular network in which the receptor tyrosine phosphatase Dlar interacts with the WRC to couple the extracellular matrix, the membrane, and the actin cytoskeleton during egg elongation. Our data uncover a mechanism by which polarity information can be transduced from a membrane receptor to a key actin regulator to control collective follicle cell migration during egg elongation. 4D-live imaging of rotating MCF10A mammary acini further suggests an evolutionary conserved mechanism driving rotational motions in epithelial morphogenesis. |
| Databáze: | OpenAIRE |
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