Preparation of an electrochemical biosensor based on lipid membranes in nanoporous alumina
| Autor: | Sandrine Morandat, Karim El Kirat, Jean-Baptiste Largueze |
|---|---|
| Přispěvatelé: | Génie Enzymatique et Cellulaire (GEC), Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2010 |
| Předmět: |
Octoxynol
Lipid Bilayers Biosensing Techniques 02 engineering and technology Model lipid bilayer Microscopy Atomic Force 010402 general chemistry 01 natural sciences Polar membrane Polyethylene Glycols Membrane Lipids Colloid and Surface Chemistry Aluminum Oxide Membrane fluidity Organic chemistry Physical and Theoretical Chemistry Lipid bilayer Electrodes Nanoporous Chemistry Membrane structure Biological membrane Electrochemical Techniques Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences Membrane Liposomes Biophysics lipids (amino acids peptides and proteins) 0210 nano-technology Porosity Fluorescence Recovery After Photobleaching Biotechnology |
| Zdroj: | HAL Colloids and Surfaces B: Biointerfaces Colloids and Surfaces B: Biointerfaces, Elsevier, 2010, 79, pp.33-40 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2010.03.011 |
| Popis: | International audience; Model lipid bilayers are versatile tools to investigate the molecular processes occurring at the membrane level. Among the model membranes, substrate supported bilayers have attracted much interest because they are robust and they can be investigated by powerful surface sensitive techniques such as electrochemical measurements. In a biosensor, lipid films can be used not only as a support for the biological sensing elements but also as sensing elements themselves to detect molecules that are able to alter the structure and the properties of biomembranes. In this work, we have prepared a tethered lipid membrane-based biosensor able to detect the alterations of membrane structure and fluidity. This tethered lipid membrane was prepared in a nanoporous aluminium oxide that provides a high surface area and a protective environment against dewetting. The membrane contained PEG-PE lipids as hydrating, protective and tethering agents and ubiquinone which is a redox lipophilic mediator embedded within the acyl chains of the lipid bilayer. The lipid membrane was prepared inside the pores of the nanoporous support by a PEG-triggered fusion of liposomes. This sensing system was efficient to detect the alterations of lipid membranes that are induced by the addition of a commonly used non-ionic detergent: Triton X-100. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect