Functional ultrasound imaging to study brain dynamics: Application of pharmaco-fUS to atomoxetine
| Autor: | Marco Valdebenito, Ludovic Venet, Benjamin Vidal, Mathieu Charvériat, Luc Zimmer, Marine Droguerre, Franck Mouthon |
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| Přispěvatelé: | Theranexus [Lyon], Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Etude et de Recherche Multimodal Et Pluridisciplinaire en imagerie du vivant (CERMEP - imagerie du vivant), Université de Lyon-Université de Lyon-CHU Grenoble-Hospices Civils de Lyon (HCL)-CHU Saint-Etienne-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Hospices Civils de Lyon (HCL) |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Pharmaco-fUS [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging Brain activity and meditation Thalamus Atomoxetine Hydrochloride Rats Inbred WKY 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Norepinephrine reuptake inhibitor medicine Animals Ultrasonography Visual Cortex Pharmacology Adrenergic Uptake Inhibitors business.industry Dentate gyrus Atomoxetine Functional ultrasound imaging Posterior Thalamic Nuclei 3. Good health Rats 030104 developmental biology Visual cortex medicine.anatomical_structure Mechanism of action Dentate Gyrus medicine.symptom business Neuroscience 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neuropharmacology Neuropharmacology, Elsevier, 2020, 179, pp.108273. ⟨10.1016/j.neuropharm.2020.108273⟩ |
| ISSN: | 1873-7064 0028-3908 |
| DOI: | 10.1016/j.neuropharm.2020.108273⟩ |
| Popis: | International audience; Highlights• A first evaluation of the potential of fUS during a pharmacological challenge.• ATX modulates cerebral blood volume in areas associated with visual processing.• fUS as a new step to move forwards new drugs to development in neurological disorders.Functional ultrasound (fUS) is a new tool enabling the imaging of brain activity through the regional monitoring of cerebral blood volume (CBV) dynamics. This innovative technique has not yet demonstrated its full potential in pharmacological applications and drug development. In the current proof-of-concept study, the impact of atomoxetine (ATX), a potent norepinephrine reuptake inhibitor and non-stimulant treatment marketed in attention-deficit/hyperactivity-disorder, was evaluated in anesthetized rat using pharmacological functional ultrasound (pharmaco-fUS) at increasing doses (0.3, 1 and 3 mg/kg). Using regions of interest (acute changes of CBV and functional connectivity) or pixel-based (general linear modeling and independent component analysis) analysis, we here demonstrated that ATX consistently displayed a hemodynamic effect in the visual cortex, the dentate gyrus and thalamus, especially visual areas such as lateral posterior thalamic nuclei and lateral geniculate nuclei (LGN). The time profile of ATX effects was dose-dependent, with fastest CBV increases at the highest dose, and longer CBV increases at the intermediate dose. Standardizing the use of pharmaco-fUS could improve our understanding of the mechanism of action of drugs active in the brain and might constitute a new step to move forward in drug development for neurological disorders. |
| Databáze: | OpenAIRE |
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