Anticancer and antimetastatic activity of Hypomyces chrysospermus, a cosmopolitan parasite in different human cancer cells

Autor: Göksu Ceylan, Zerrin Cantürk, Ayşe Betül Karaduman, Miriş Dikmen, Selin Engür Öztürk, Mustafa Yamaç
Přispěvatelé: Anadolu Üniversitesi
Rok vydání: 2020
Předmět:
0301 basic medicine
cell migration
Cell
drug response
antioxidant activity
IC50
MCF-7 cell line
Pharmacology
Antioxidants
apoptotic protease activating factor 1
0302 clinical medicine
Cell Movement
Cytotoxic T cell
HLF cell line (lung fibroblast)
acetic acid ethyl ester
antineoplastic agent
cancer cell
biology
Hypomyces chrysospermus
messenger RNA
drug effect
fungus
NF-kappa B
Biological activity
General Medicine
cell invasion
unclassified drug
Anticancer
immunoglobulin enhancer binding protein
medicine.anatomical_structure
real time polymerase chain reaction
A-549 cell line
030220 oncology & carcinogenesis
Hypocreales
MCF-7 Cells
cytotoxicity
Tumor necrosis factor alpha
Antioxidant
antiproliferative activity
tumor necrosis factor
Antineoplastic Agents
antineoplastic activity
WST-1 assay
Article
03 medical and health sciences
Caco-2 cell line
Cell Line
Tumor
drug mechanism
Genetics
medicine
Humans
controlled study
human
Molecular Biology
Hypomyces chrysospermus extract
Plant Extracts
Tumor Necrosis Factor-alpha
Basidiomycota
clinical effectiveness
human cell
CCD-841CoN cell line
fungal extract
Antimetastatic
CCD 19 Lu cell line
biology.organism_classification
Antineoplastic Agents
Phytogenic
drug efficacy
Apoptotic Protease-Activating Factor 1
cell proliferation
030104 developmental biology
A549 Cells
Cell culture
Cancer cell
cyclophosphamide
Caco-2 Cells
Zdroj: Molecular Biology Reports. 47:3765-3778
ISSN: 1573-4978
0301-4851
Popis: WOS: 000530769000001
PubMed: 32378168
The importance of microbial natural compounds in drug research is increasing every year and they are used to prevent or treat a variety of diseases. Hypomyces chrysospermus is a cosmopolitan parasite of many Boletaceae members. Since not much work has been conducted to date, this study is undertaken to explore the anticancer effect, including the antiproliferative and antimetastatic activity of Hypomyces chrysospermus. the aim of this study is to determine the antiproliferative and antimetastatic activity of Hypomyces chrysospermus ethyl acetate extract, having antioxidant activity, against A549, Caco2, MCF-7 human cancer and CCD-19 Lu and CCD 841 CoN healthy human cell lines. Firstly, cytotoxic activity was determined by the WST-1 assay. After cell proliferations and anti-metastatic effects were investigated by a real-time cell analysis system (RTCA-DP) and IC50 concentrations were calculated for each cell line. in addition, the expression levels of Apaf-1, TNF and NF-kB mRNA in cancer cells were investigated with RealTime-PCR. the ethyl acetate extract of Hypomyces chrysospermus presented anticancer activities including antiproliferative and antimetastatic effects. Hypomyces chrysospermus as a source of biologically active metabolites can be used as an important resource in the development of new anticancer effective agents.
Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113Z746]
This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant No. 113Z746. the authors extend their thanks to Dr. Bukay Yenice Gursu for technical contribution of the dendrogram.
Databáze: OpenAIRE
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