Study of Genetic Stability of Human Bone Marrow Multipotent Mesenchymal Stromal Cells
| Autor: | V. A. Nikitina, E. V. Skorobogatova, N. P. Bochkov, T. V. Shamanskaya, E. Yu. Osipova, S. A. Rumyantsev, L. D. Katosova |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Monosomy Stromal cell Aneuploidy Bone Marrow Cells Biology General Biochemistry Genetics and Molecular Biology Immunophenotyping medicine Humans Cells Cultured Cell Proliferation Chromosome Aberrations Multipotent Stem Cells Mesenchymal stem cell Genetic Variation Cell Differentiation Mesenchymal Stem Cells Karyotype General Medicine medicine.disease Molecular biology Phenotype medicine.anatomical_structure Karyotyping Female Bone marrow Trisomy |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 150:627-631 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-011-1207-1 |
| Popis: | Immunophenotype, proliferation rate, and genetic stability parameters of bone marrow multipotent mesenchymal stromal cells were studied. Despite the reduction of proliferative activity by passages 11-12, the cells retained the characteristic immunophenotype. The incidence of spontaneous aneuploidy for autosomes 6, 8, 11 and sex chromosomes was evaluated. Two cultures of mesenchymal stromal cells carrying aneuploid cell clones were detected: with chromosome 8 trisomy and X chromosome monosomy. The results indicate the possibility of genetic transformation and selection of mesenchymal stromal cells with abnormal karyotype during in vitro culturing. |
| Databáze: | OpenAIRE |
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