The temperature-regulated DEAD-box RNA helicase CrhR interactome: Autoregulation and photosynthesis-related transcripts
| Autor: | Florian Heyl, Bruno Huettel, Wolfgang R. Hess, Anzhela Migur, Janina Fuss, George W. Owttrim, Claudia Steglich, Afshan Srikumar, Rolf Backofen, Jogadhenu S. S. Prakash, Richard Reinhardt |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Regulation of gene expression 0303 health sciences Messenger RNA DEAD box Physiology Synechocystis RNA Plant Science Biology biology.organism_classification 01 natural sciences RNA Helicase A Interactome Cell biology 03 medical and health sciences Gene family 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Journal of experimental botany. |
| ISSN: | 1460-2431 |
| Popis: | RNA helicases play crucial functions in RNA biology. In plants, RNA helicases are encoded by large gene families, performing roles in abiotic stress responses, development, the post-transcriptional regulation of gene expression as well as house-keeping functions. Several of these RNA helicases are targeted to the organelles, mitochondria and chloroplasts. Cyanobacteria are the direct evolutionary ancestors of plant chloroplasts. The cyanobacterium Synechocystis 6803 encodes a single DEAD-box RNA helicase, CrhR, that is induced by a range of abiotic stresses, including low temperature. Though the ΔcrhR mutant exhibits a severe cold-sensitive phenotype, the physiological function(s) performed by CrhR have not been described. To identify transcripts interacting with CrhR, we performed RNA co-immunoprecipitation with extracts from a Synechocystis crhR deletion mutant expressing the FLAG-tagged native CrhR or a K57A mutated version with an anticipated enhanced RNA binding. The composition of the interactome was strikingly biased towards photosynthesis-associated and redox-controlled transcripts. A transcript highly enriched in all experiments was the crhR mRNA, suggesting an auto-regulatory molecular mechanism. The identified interactome explains the described physiological role of CrhR in response to the redox poise of the photosynthetic electron transport chain and characterizes CrhR as an enzyme with a diverse range of transcripts as molecular targets.HighlightThe cyanobacterial DEAD-box RNA helicase CrhR binds mainly photosynthesis-associated and redox-controlled transcripts connecting its regulation, localization and phenotypes of mutants for the first time with a set of potential RNA targets. |
| Databáze: | OpenAIRE |
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