Gene families encoding 11S globulin and 2S albumin isoforms of jelly fig (Ficus awkeotsang) Achenes
| Autor: | Anna C. N. Chua, Li-Jen Lin, Wing-Ming Chou, Jason T.C. Tzen, Eric S. L. Hsiao, Yuan-Chang Yang |
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| Rok vydání: | 2008 |
| Předmět: |
Gene isoform
Achene DNA Complementary Globulin Molecular Sequence Data Genes Plant Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Complementary DNA Albumins Storage protein Gene family Amino Acid Sequence Molecular Biology Peptide sequence chemistry.chemical_classification Expressed Sequence Tags Expressed sequence tag biology Sequence Homology Amino Acid Organic Chemistry Globulins General Medicine Ficus chemistry Multigene Family Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Electrophoresis Polyacrylamide Gel Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 72(2) |
| ISSN: | 1347-6947 |
| Popis: | Seed storage proteins of plants commonly comprise several groups of multiple isoforms encoded by gene families. From about 300 expressed sequence tag (EST) clones in maturing jelly fig (Ficus awkeotsang Makino) achenes, gene families encoding precursor polypeptides of two storage protein classes, including six 11S globulin isoforms and two 2S albumin isoforms, were identified. Complete sequences encoding the precursor polypeptides of these eight storage proteins were obtained by sequencing the pertinent EST clones that contained full-length cDNA fragments. Matrix-assisted laser desorption/ionization mass spectrometry analysis confirmed the presence of these storage protein isoforms in the extract of jelly fig achenes resolved in SDS-PAGE. The amino acid compositions of the deduced storage proteins indicated that achene proteins in jelly fig are nutritive, for both isoforms of 2S albumin are sulfur-rich, and one of them is also rich in tryptophan. |
| Databáze: | OpenAIRE |
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