Real-time hexose monophosphate shunt activity in light- and dark-adapted rabbit retinas
| Autor: | Eric Aguiar, Dominic Man-Kit Lam, Hong-Ming Cheng |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
genetic structures
Light Dark Adaptation Pentose phosphate pathway Retina Pentose Phosphate Pathway Cellular and Molecular Neuroscience tert-Butylhydroperoxide medicine Animals Lagomorpha biology Chemistry Rabbit (nuclear engineering) General Medicine biology.organism_classification Adaptation Physiological Sensory Systems Peroxides Dark-adapted Ophthalmology medicine.anatomical_structure Biochemistry sense organs Rabbits |
| Zdroj: | Ophthalmic research. 19(5) |
| ISSN: | 0030-3747 |
| Popis: | Correlation proton nuclear magnetic resonance spectroscopy was performed on light- and dark-adapted rabbit retinas to elucidate real-time hexose monophosphate shunt (HMPS) activity. Light significantly stimulated retinal HMPS initially, which consumed 17% more glucose than that in dark-adapted retinas. The glucose consumption eventually declined to 16% of total, the baseline level, after 60 min of light exposure. In contrast, dark-adapted retinas showed an initial HMPS activity of 29% total glucose consumption, which declined to the basal level after 40 min. Lactate production appeared stable in both sets of retinas. Tert-butyl hydroperoxide (1 mM) also stimulated the shunt; however, a combination of light-adaptation and tert-butyl hydroperoxide did not stimulate the shunt additively. These data indicate that the retina has limited HMPS capacity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|