Evaluation of a Chemiluminescent Immunoassay for Measurement of Equine Insulin
| Autor: | Catherine M. McGowan, Gina Pinchbeck, H.B. Carslake |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Serial dilution Laminitis 040301 veterinary sciences medicine.medical_treatment Coefficient of variation Serum insulin Radioimmunoassay Standard Article Horse Sensitivity and Specificity 0403 veterinary science Endocrinology Chemiluminescent immunoassay Internal medicine medicine Animals Insulin Horses High concentration Immunoassay Chromatography General Veterinary business.industry 0402 animal and dairy science Reproducibility of Results 04 agricultural and veterinary sciences Serum samples 040201 dairy & animal science Standard Articles Luminescent Measurements ELISA Metabolic EQUID business |
| Zdroj: | Journal of Veterinary Internal Medicine |
| ISSN: | 1939-1676 0891-6640 |
| Popis: | Background Many diagnostic tests for insulin dysregulation use reference intervals established with an insulin radioimmunoassay (RIA) that is no longer available. A chemiluminescent immunoassay (CLIA) is commonly used for the measurement of serum insulin concentration in clinical practice but requires further validation, especially at clinically relevant reference intervals. Objectives To evaluate the CLIA for measurement of equine insulin and compare it to the previously validated, but now unavailable RIA. Samples Equine serum samples (n = 78) from clinical and experimental studies. Methods In this experimental study, performance of the CLIA was evaluated using standard variables, including comparison with the RIA. Continuous and binary outcomes were analyzed. Results The CLIA showed good intra-assay (coefficient of variation [CV], 1.8–2.4%) and interassay (CV, 3–7.1%) precision. Acceptable recovery on dilution (100 ± 10%) was achieved only at dilutions |
| Databáze: | OpenAIRE |
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