The mouse gene Ptprf encoding the leucocyte common antigen-related molecule LAR: cloning, characterization and chromosomal localization

Autor: Schaapveld, R.Q.J., Maagdenberg, A.M.J.M. van den, Schepens, J.T.G., Olde Weghuis, D.E.M., Geurts van Kessel, A.H.M., Streuli, M., Wieringa, B., Hendriks, W.J.A.J.
Rok vydání: 1995
Předmět:
Molecular Sequence Data
analyse van de functie van LAR fosfatase domeinen middels exon-gerichte mutagenese [De celbiologische rol van proteïne-tyrosine fosfatases]
Receptors
Cell Surface
Protein tyrosine phosphatase
PTPRF
PTPRC
Homology (biology)
Exon
Mice
Mapping of cloned genes and DNA fragments by means of somatic cell hybrids and (interphase) in situ hybridization
Species Specificity
Genetics
Animals
Humans
Amino Acid Sequence
Gene
GeneralLiterature_REFERENCE(e.g.
dictionaries
encyclopedias
glossaries)
Phylogeny
biology
Base Sequence
Kartering van gekloneerde genen en DNA-fragmenten met behulp van somatische celhybriden en (interfase-) in situ hybridisatie
Receptor-Like Protein Tyrosine Phosphatases
Class 4
Chromosome Mapping
Membrane Proteins
Molecular biology
Protein Structure
Tertiary
Rats
genomic DNA
Receptor-Like Protein Tyrosine Phosphatases
Genes
Multigene Family
biology.protein
Protein Tyrosine Phosphatases
functional analysis of LAR phosphatases domains by exon-directed mutagenesis [The celbiological role of protein-tyrosine phosphatases]
Signal Transduction
Zdroj: Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes, 27, pp. 124-130
Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes, 27, 124-130
Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes, 27, 1, pp. 124-130
Genomics : International Journal of Gene Mapping and Nucleotide Sequencing Emphasizing Analyses of the Human and Other Complex Genomes, 27, 124-130. Academic press inc jnl-comp subscriptions
ISSN: 0888-7543
DOI: 10.1006/geno.1995.1014
Popis: The human receptor-like protein tyrosine phosphatase leukocyte common antigen-related molecule (LAR; gene symbol PTPRF) closely resembles cell adhesion molecules, which suggests that it may be involved in the regulation of phosphotyrosine levels through cell-cell or cell-matrix interactions. To obtain a better understanding of LAR function, we have characterized the mouse Ptprf gene as a first step toward site-directed mutagenesis studies in vitro and in vivo. The cytoplasmic region of the mouse LAR (mLAR) protein is encoded by 11 exons that span only 4.5 kb of genomic DNA. Compared to the known exon-intron structures of other mammalian receptor-like protein tyrosine phosphatase genes, such as Ptpra (encoding LRP) and Ptprc (coding for Ly-5), the Ptprf gene part encoding the cytoplasmic region of mLAR contains not only smaller, but also fewer introns. Sequence analysis of both phosphatase domains of mLAR and its homologs MPTPδ and mRPTP σ revealed a higher evolutionary conservation of the second, C-terminal domain in comparison to the first domain. Fluorescence in situ hybridization was used to map the Ptprf gene to region C6-D1 on mouse chromosome 4.
Databáze: OpenAIRE
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