Acacetin ameliorates insulin resistance in obesity mice through regulating Treg/Th17 balance via MiR-23b-3p/NEU1 Axis
| Autor: | Xueyang Wang, Xiaoqian Liu, Zhiping Peng, Yan Wei, Jianhong Jing |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty MiR-23b-3p Endocrinology Diabetes and Metabolism Neuraminidase Inflammation lcsh:Diseases of the endocrine glands. Clinical endocrinology Acacetin T cells/T helper 17 cells Flow cytometry Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Insulin resistance Downregulation and upregulation Western blot Neuraminidase 1 Internal medicine medicine Animals Obesity lcsh:RC648-665 medicine.diagnostic_test business.industry General Medicine medicine.disease Flavones Mice Inbred C57BL MicroRNAs 030104 developmental biology Endocrinology chemistry 030220 oncology & carcinogenesis Th17 Cells Tumor necrosis factor alpha medicine.symptom business Research Article |
| Zdroj: | BMC Endocrine Disorders BMC Endocrine Disorders, Vol 21, Iss 1, Pp 1-12 (2021) |
| ISSN: | 1472-6823 |
| Popis: | Background The role of miR-23b-3p in insulin resistance (IR) remained poorly understood. Methods After acacetin injection, obesity-induced IR model was constructed with or without miR-23b-3p upregulation and Neuraminidase 1 (NEU1) overexpression in mice. Body weight, serum metabolite and fat percent of the mice were measured. Tests on oral glucose and insulin tolerance were performed, and inflammatory cytokines C-reactive protein (CRP), Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and monocyte chemoattractant protein 1 (MCP1) levels were quantified with enzyme-linked immunosorbent assay (ELISA). The binding sites between miR-23b-3p and NEU1 were predicted by TargetScan, and verified using dual-luciferase reporter assay. Relative expressions were detected with quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Proportion of Treg and Th17 cells in total CD4+ T cells was detected with flow cytometry. Results MiR-23b-3p offset the effects of acacetin on body weight, fat percent, inflammatory cytokines levels and expressions of markers of regulatory T cells (Treg cells) and T helper 17 cells (Th17 cells), NEU1 and miR-23b-3p. NEU1 was a target of miR-23b-3p, and overexpressed NEU1 reversed the effects of upregulated miR-23b-3p on reducing Treg cells but increased body weight, fat percent and inflammatory cytokines levels, percentage of Th17 cells, and upregulated NEU1 expression. Conclusion Upregulation of miR-23b-3p offset the effects of acacetin on obesity-induced IR through regulating Treg/Th17 cell balance via targeting NEU1.The present findings provide a possible prevention strategy for obesity-induced IR. |
| Databáze: | OpenAIRE |
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