Rapid ex vivo assessment of cancer prognosis by fluorescence imaging of nucleolus using nitrogen doped carbon dots
| Autor: | Dan Wang, Pengfei Jiang, Bobo Gu, Shihui Ding, Xunbin Wei, Wen Pang |
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| Rok vydání: | 2020 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Nucleolus Nitrogen Cancer therapy Nitrogen doped 02 engineering and technology 01 natural sciences Biochemistry Fluorescence Analytical Chemistry Cancer prognosis Neoplasms Quantum Dots medicine Environmental Chemistry Humans Spectroscopy Fluorescent Dyes Chemistry 010401 analytical chemistry Optical Imaging Cancer 021001 nanoscience & nanotechnology medicine.disease Prognosis Carbon 0104 chemical sciences Spectrometry Fluorescence Cancer cell Cancer research 0210 nano-technology Ex vivo |
| Zdroj: | Analytica chimica acta. 1154 |
| ISSN: | 1873-4324 |
| Popis: | Cancer severely threatens human health currently, promoting the rapid development of cancer treatment strategies. In addition to cancer therapy, assessment of cancer prognosis, which can evaluate the success with treatment and chances of recovery as well as assist to make subsequent therapeutic schedule, is also remarkably indispensable and important. Conventional technologies can’t provide rapid and highly-sensitive assessment of cancer prognosis at cytological level. Herein, an effective nitrogen doped carbon dots with intrinsic nucleolus-targeting capability and high fluorescence quantum yield are synthesized, characterized and employed for fluorescence imaging of nucleolus, which is closely related to the biological alteration of cancer cell. The cancer prognosis thus can be accurately (limit of detection: 50 nM) and rapidly (5 min) assessed at subcellular organelle level from nucleolar characteristics, which are visualized and analyzed by the captured fluorescence images. Outstanding assessment performance endows the proposed technology with great potential for future clinical research. |
| Databáze: | OpenAIRE |
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